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Titolo:
Determination of risperidone and its major metabolite 9-hydroxyrisperidonein human plasma by reversed-phase liquid chromatography with ultraviolet detection
Autore:
Avenoso, A; Facciola, G; Salemi, M; Spina, E;
Indirizzi:
Univ Messina, Policlin Univ, Sch Med, Inst Pharmacol, I-98125 Messina, Italy Univ Messina Messina Italy I-98125 nst Pharmacol, I-98125 Messina, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 746, anno: 2000,
pagine: 173 - 181
SICI:
1387-2273(20000915)746:2<173:DORAIM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCHEMICAL DETECTION; PERFORMANCE; PHARMACOKINETICS; SERUM; VARIABILITY; 2D6;
Keywords:
risperidone; 9-hydroxyrisperidone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Spina, E Univ Messina, Policlin Univ, Sch Med, Inst Pharmacol, Via Consolare Valeria, I-98125 Messina, Italy Univ Messina Via Consolare Valeria Messina Italy I-98125 , Italy
Citazione:
A. Avenoso et al., "Determination of risperidone and its major metabolite 9-hydroxyrisperidonein human plasma by reversed-phase liquid chromatography with ultraviolet detection", J CHROMAT B, 746(2), 2000, pp. 173-181

Abstract

A simple and sensitive high-performance Liquid chromatographic (HPLC) method with UV absorbance detection is described for the quantitation of risperidone and its major metabolite 9-hydroxyrisperidone in human plasma, using clozapine as internal standard. After sample alkalinization with 1 ml of NaOH (2 M) the test compounds were extracted from plasma using diisopropyl ether-isoamylalcohol (99:1, v/v). The organic phase was back-extracted with 150 mu l potassium phosphate (0.1 M, pH 2.2) and 60 mu l of the acid solution was injected into a C-18 BDS Hypersil analytical column (3 mu m, 100x4.6 mm I.D.). The mobile phase consisted of phosphate buffer (0.05 M, pH 3.7 with 25% H3PO4)-acetonitrile (70:30, v/v), and was delivered at a flow-rate of 1.0 ml/min. The peaks were detected using a UV detector set at 278 nm andthe total time for a chromatographic separation was about 4 min. The method was validated for the concentration range 5-100 ng/ml. Mean recoveries were 98.0% for risperidone and 83.5% for 9-hydroxyrisperidone. Intra- and inter-day relative standard deviations were less than 11% for both compounds, while accuracy, expressed as percent error, ranged from 1.6 to 25%. The limit of quantitation was 2 ng/ml for both analytes. The method shows good specificity with respect to commonly prescribed psychotropic drugs, and it hassuccessfully been applied for pharmacokinetic studies and therapeutic drugmonitoring. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 05:57:02