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Titolo:
Ligand-induced internalization of neurotensin in transfected COS-7 cells: differential intracellular trafficking of ligand and receptor
Autore:
Vandenbulcke, F; Nouel, D; Vincent, JP; Mazella, J; Beaudet, A;
Indirizzi:
McGill Univ, Montreal Neurol Inst, Montreal, PQ H2A 2B4, Canada McGill Univ Montreal PQ Canada H2A 2B4 Inst, Montreal, PQ H2A 2B4, Canada Univ Nice, CNRS, Inst Pharmacol Mol, F-06560 Valbonne, France Univ Nice Valbonne France F-06560 harmacol Mol, F-06560 Valbonne, France
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 17, volume: 113, anno: 2000,
pagine: 2963 - 2975
SICI:
0021-9533(200009)113:17<2963:LIONIT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETROGRADE AXONAL-TRANSPORT; PROTEIN-COUPLED RECEPTOR; EPIDERMAL GROWTH-FACTOR; COATED PIT FORMATION; MEDIATED ENDOCYTOSIS; THIMET OLIGOPEPTIDASE; FUNCTIONAL EXPRESSION; TRANSFERRIN RECEPTOR; DOPAMINERGIC-NEURONS; CONFOCAL MICROSCOPY;
Keywords:
neurotensin; NT1; internalization; trafficking; confocal microscopy; trans-Golgi network;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Beaudet, A McGill Univ, Montreal Neurol Inst, Montreal, PQ H2A 2B4, CanadaMcGill Univ Montreal PQ Canada H2A 2B4 eal, PQ H2A 2B4, Canada
Citazione:
F. Vandenbulcke et al., "Ligand-induced internalization of neurotensin in transfected COS-7 cells: differential intracellular trafficking of ligand and receptor", J CELL SCI, 113(17), 2000, pp. 2963-2975

Abstract

The neuropeptide neurotensin (NT) is known to be internalized in a receptor-mediated fashion into its target cells. To gain insight into the mechanisms underlying this process, we monitored in parallel the migration of the NT1 neurotensin receptor subtype and a fluorescent analog of NT (fluo-NT) inCOS-7 cells transfected with a tagged NT1 construct. Fluo-NT internalization was prevented by hgpertonic sucrose, potassium depletion and cytosol acidification, demonstrating that it proceeded via clathrin-coated pits, Within 0-30 minutes, fluo-NT accumulated together with its receptor in Acridine Orange-positive, acidic organelles, These organelles concentrated transferrin and immunostained positively for rab 5A, therefore they were early endosomes, After 30-45 minutes, the ligand and its receptor no longer colocalized, Fluo-NT was first found in rab 7-positive late endosomes and later in a nonacidic juxtanuclear compartment identified as the Trans-Golgi Network (TGN) by virtue of its staining for syntaxin 6. This juxtanuclear compartmentalso stained positively for rab 7 and for the TGN/pericentriolar recyclingendosome marker rab 11, suggesting that the ligand could have been recruited to the TGN from either late or recycling endosomes, By that time, internalized receptors were detected in Lamp-l-immunoreactive lysosomes, These results demonstrate that neurotensin/NT1 receptor complexes follow a recycling cycle that is unique among the G protein-coupled receptors studied to date, and provide the first evidence for the targeting of a nonendogenous protein from endosomes to the TGN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:25:06