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Titolo:
Monoclonal antibodies generated against recombinant ATM support kinase activity
Autore:
Alligood, KJ; Milla, M; Rhodes, N; Ellis, B; Kilpatrick, KE; Lee, A; Gilmer, TM; Lansing, TJ;
Indirizzi:
Glaxo Wellcome Inc, Res & Dev, Dept Canc Biol, Res Triangle Pk, NC 27709 USA Glaxo Wellcome Inc Res Triangle Pk NC USA 27709 Triangle Pk, NC 27709 USA Glaxo Wellcome Inc, Res & Dev, Dept Prot Sci, Res Triangle Pk, NC 27709 USA Glaxo Wellcome Inc Res Triangle Pk NC USA 27709 Triangle Pk, NC 27709 USA
Titolo Testata:
HYBRIDOMA
fascicolo: 4, volume: 19, anno: 2000,
pagine: 317 - 321
SICI:
0272-457X(200008)19:4<317:MAGARA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATAXIA-TELANGIECTASIA; IONIZING-RADIATION; RAPID PRODUCTION; DNA-DAMAGE; PHOSPHORYLATION; P53;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Lansing, TJ Glaxo Wellcome Inc, Res & Dev, Dept Canc Biol, 5 Moore Dr, ResTriangle Pk, NC 27709 USA Glaxo Wellcome Inc 5 Moore Dr Res Triangle Pk NCUSA 27709 USA
Citazione:
K.J. Alligood et al., "Monoclonal antibodies generated against recombinant ATM support kinase activity", HYBRIDOMA, 19(4), 2000, pp. 317-321

Abstract

We report on the rapid generation of two monoclonal antibodies, ATM A16.35and ATM. D16.11, that bind to the kinase domain of mutated ataxia telangiectasia (ATM). These antibodies were generated against E. coli-expressed recombinant protein using the RIMMS strategy. We show that ATM A16.35 binds ATM by Western blot analysis, and ATM D16.11 forms immune complexes with native ATM in immunoprecipitations without neutralizing kinase activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 05:36:24