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Titolo:
DIFFERENTIAL MODULATION OF LAK AND ADCC FUNCTIONS OF NATURAL-KILLER-CELLS FROM AK-5 TUMOR-BEARING RATS BY IL-2, IL-12 AND IFN-GAMMA
Autore:
MURALIKRISHNA K; VARALAKSHMI C; KHAR A;
Indirizzi:
CTR CELLULAR & MOL BIOL,UPPAL RD HYDERABAD 500007 ANDHRA PRADESH INDIA CTR CELLULAR & MOL BIOL HYDERABAD 500007 ANDHRA PRADESH INDIA
Titolo Testata:
Cytokines cellular & molecular therapy
fascicolo: 1, volume: 3, anno: 1997,
pagine: 51 - 58
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUS IMMUNOLOGICAL REGRESSION; MEDIATED CYTOTOXICITY; NK CELLS; ADHESION MOLECULES; CYTO-TOXICITY; HISTIOCYTOMA; EXPRESSION; LYMPHOKINE; BLOOD; INTERLEUKIN-12;
Keywords:
INTERLEUKINS; NK CELL FUNCTION; LAK; ADCC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
K. Muralikrishna et al., "DIFFERENTIAL MODULATION OF LAK AND ADCC FUNCTIONS OF NATURAL-KILLER-CELLS FROM AK-5 TUMOR-BEARING RATS BY IL-2, IL-12 AND IFN-GAMMA", Cytokines cellular & molecular therapy, 3(1), 1997, pp. 51-58

Abstract

Modulation of NK cell activity by various lymphokines is well documented. Freshly isolated NK cells exhibit natural cytotoxicity (NC) and antibody-dependent cellular cytotoxicity (ADCC) against certain targets. Upon stimulation with IL-2, the NK cells acquire lymphokine-activated killer (LAK) activity, which enables them to kill a wide variety of targets. Our results demonstrate that freshly isolated NH cells from tumor-bearing rats exhibit NH activity towards YAC-1, and ADCC activitytowards AH-5 targets. fresh NH cells did not lyse AH-5 targets in theabsence of anti AH-5 antibody. However, in vitro culture of fresh NK cells in the presence of rat recombinant IL-2 (r-IL-2) over a period of 7 days resulted in complete loss of ADCC activity with concomitant acquisition of LAH activity on AK-5 tumor targets. The LAH and ADCC activities could be effectively distinguished based on our observation that monoclonal anti-rat ICAM-1 and anti-rat LFA-1 antibodies inhibited only LAK activity, but not ADCC activity, in addition, anti-AK-5 antiserum, but not purified anti-AK-5 antibody, inhibited LAK activity, suggesting that soluble ICAM-1 present in hyperimmune serum could be responsible for inhibition of LAK activity. The loss of ADCC activity uponculture of NK cells with rIL-2 was not observed when the cultures were grown on a macrophage feeder layer. In addition, NK cells retained their ADCC activity when cultures were supplemented with a combination of recombinant IL-2 and IL-12. However IL-2, IFN-gamma or IL-12 alone did not prevent the loss of ADCC activity, suggesting the requirement of a combination of these lymphokines for the maintainance of ADCC activity.

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Documento generato il 13/07/20 alle ore 17:35:23