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Titolo:
FLOW CYTOMETRIC ASSAY FOR IN-VIVO GENOTOXIC EFFECTS OF PESTICIDES IN GREEN FROGS (RANA-CLAMITANS)
Autore:
LOWCOCK LA; SHARBEL TF; BONIN J; OUELLET M; RODRIGUE J; DESGRANGES JL;
Indirizzi:
MAX PLANCK INST VERHALTENSPHYSIOL,ARBEITGRP MICHIELS D-82319 SEEWIESEN GERMANY MCGILL UNIV,REDPATH MUSEUM MONTREAL PQ H3A 2K6 CANADA CANADIAN WILDLIFE SERV ST FOY PQ G1V 4H5 CANADA
Titolo Testata:
Aquatic toxicology
fascicolo: 4, volume: 38, anno: 1997,
pagine: 241 - 255
SICI:
0166-445X(1997)38:4<241:FCAFIG>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIATION-INDUCED MICRONUCLEI; CHROMOSOME-ABERRATIONS; XENOPUS-LAEVIS; DNA DAMAGE; SALAMANDERS; ERYTHROCYTES; CATESBEIANA; TADPOLES; SCRIPTA; GENOME;
Keywords:
FLOW CYTOMETRY; PESTICIDE; AMPHIBIAN; GENOTOXICITY; ANEUPLOIDY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
L.A. Lowcock et al., "FLOW CYTOMETRIC ASSAY FOR IN-VIVO GENOTOXIC EFFECTS OF PESTICIDES IN GREEN FROGS (RANA-CLAMITANS)", Aquatic toxicology, 38(4), 1997, pp. 241-255

Abstract

Frogs from farming regions in Quebec suffer a suite of physical and physiological problems associated with the use of agricultural pesticides. Flow cytometry was used to compare incidence of abnormal DNA profile, half-peak coefficient of variation (CV), and variation in genome size (pg DNA per haploid nucleus) between Green frogs (Rana clamitans) from such fanning areas (corn and potato fields) and control sites dissociated from agricultural practices, to infer possible genomic manifestations of pesticide use. There was a significant increase in abnormal DNA profile in individuals from corn fields relative to the control sites (P<0.05). In all comparisons, adult frogs showed greater CVs than did juveniles (P<0.0001), Among adults, CVs were higher for samples taken from both potato and corn fields relative to the control samples(P<0.005), while in juveniles, only individuals from corn plots showed elevated CVs relative to controls (P<0.05). Juveniles showing physical deformity had significantly higher CVs than normal individuals (P<0.05), although there were no similar correlations with physiological disruption. Mean C-value (variation in DNA content) was different between adults and juveniles in all treatments (P<0.0001), but there were no significant differences in mean C-value and variance of such among similar age classes between treatments. The different classes of DNA damage found in this study are reflective of either acute or cumulative pesticide toxicity, and are exhibited by both sick and apparently normal individuals. We therefore believe flow cytometry to be a powerful technique for the measurement of pesticide-induced genomic disruption in amphibian populations. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 03:29:16