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Titolo:
Multi-lineage expansion potential of primitive hematopoietic progenitors: Superiority of umbilical cord blood compared to mobilized peripheral blood
Autore:
Lewis, ID; Verfaille, CM;
Indirizzi:
Univ Minnesota, Stem Cell Inst, Dept Med, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 pt Med, Minneapolis, MN 55455 USA Univ Minnesota, Ctr Canc, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 r Canc, Minneapolis, MN 55455 USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 9, volume: 28, anno: 2000,
pagine: 1087 - 1095
SICI:
0301-472X(200009)28:9<1087:MEPOPH>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM CULTURE; COLONY-STIMULATING FACTOR; EX-VIVO EXPANSION; LIMITING DILUTION ASSAYS; MURINE BONE-MARROW; KILLER NK CELLS; STEM-CELLS; IN-VITRO; INITIATING CELLS; SELF-RENEWAL;
Keywords:
hematopoiesis; AFT024; expansion; multi-lineage; cord blood;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Verfaille, CM Box 806 UMHC,420 Delaware St SE, Minneapolis, MN 55455 USA Box 806 UMHC,420 Delaware St SE Minneapolis MN USA 55455 SA
Citazione:
I.D. Lewis e C.M. Verfaille, "Multi-lineage expansion potential of primitive hematopoietic progenitors: Superiority of umbilical cord blood compared to mobilized peripheral blood", EXP HEMATOL, 28(9), 2000, pp. 1087-1095

Abstract

Objective. The majority of studies assessing ex-vivo expansion of primitive hematopoietic cells only address production of myeloid progeny whereas itmay be more appropriate to maintain or expand progenitors that retain capacity for multilineage differentiation. In this study, we assessed the capacity of the murine fetal liver cell line AFT024 to expand primitive myeloid progenitors (LTC-IC) and lymphoid progenitors (NK-IC) from umbilical cord blood (CB) and mobilized peripheral blood (PB) CD34(+)lin(-)38(-) cells. Methods, Sorted cells were established in expansion cultures in direct contact with the feeder or in a transwell above the feeder (noncontact culture) and various combinations of Flt-3L (FL), stem cell factor, interleukin 7,thrombopoietin (Tpo), and macrophage inflammatory protein-1 alpha added. Frequency of LTC-IC and NK-IC was assessed at day 0 and following 2 and 5 weeks expansion culture,Results. CB contained significantly more LTC-IC and NK-IC at day 0 and showed an enhanced capacity for expansion compared to PB, The combination of FL and Tpo showed maximal expansion of CB LTC-IC and NK-IC at 5 weeks in both contact and noncontact conditions. In contrast, expansion of PB LTC-IC and NK-IC was maximal at 2 weeks and required multiple cytokines,Conclusions, These results demonstrate that AFT024 can expand primitive hematopoietic progenitors from CB and PB and expanded cells retain the capacity for myeloid and lymphoid differentiation. These findings emphasize the importance of assessing multi-lineage differentiation capacity following ex-vivo expansion. Elucidation of specific factors necessary for ex-vivo expansion will contribute to the development of a clinically applicable system. (C) 2000 International Society for Experimental Hematology. Published by Elsevier Science Inc.

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Documento generato il 01/04/20 alle ore 20:47:57