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Titolo:
Differences in megakaryocyte expansion potential between CD34(+) stem cells derived from cord blood, peripheral blood, and bone marrow from adults and children
Autore:
van den Oudenrijn, S; von dem Borne, AEGK; de Haas, M;
Indirizzi:
Netherlands Blood Transfus Serv, CLB, Cent Lab, NL-1105 AZ Amsterdam, Netherlands Netherlands Blood Transfus Serv Amsterdam Netherlands NL-1105 AZ erlands Univ Amsterdam, Acad Med Ctr, Lab Expt & Clin Immunol, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ Z Amsterdam, Netherlands Univ Amsterdam, Acad Med Ctr, Dept Hematol, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ Z Amsterdam, Netherlands
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 9, volume: 28, anno: 2000,
pagine: 1054 - 1061
SICI:
0301-472X(200009)28:9<1054:DIMEPB>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
EX-VIVO EXPANSION; EARLY-ACTING CYTOKINES; PROGENITOR CELLS; HEMATOPOIETIC PROGENITORS; HUMAN THROMBOPOIETIN; TRANSPLANTATION; GROWTH; CULTURE; SERUM; DIFFERENTIATION;
Keywords:
megakaryocyte; expansion; bone marrow; peripheral blood; cord blood;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: de Haas, M CLB, Dept Expt Immunohematol, Plesmanlaan 125, NL-1066 CX Amsterdam, Netherlands CLB Plesmanlaan 125 Amsterdam Netherlands NL-1066 CX therlands
Citazione:
S. van den Oudenrijn et al., "Differences in megakaryocyte expansion potential between CD34(+) stem cells derived from cord blood, peripheral blood, and bone marrow from adults and children", EXP HEMATOL, 28(9), 2000, pp. 1054-1061

Abstract

Objective. Reinfusion of ex vivo expanded autologous megakaryocytes together with stem cell transplantation may be useful to prevent or reduce the period of chemotherapy-induced thrombocytopenia. We compared the megakaryocyte expansion potential of CD34(+) stem cells derived from different sources:cord blood (CB), peripheral blood (PB), bone marrow from adults (ABM), andbone marrow from children (ChBM), Three different growth factor combinations were tested to identify the best combination for each of the sources. Materials and Methods. CD34(+) cells were isolated from CB, PB, ABM, or ChBM and cultured in an in vitro liquid culture system in the presence of thrombopoietin (Tpo), Tpo + interleukin (IL-1), or Tpo + IL-3, After 8 days, proliferation was determined and the cultured cells were identified with lineage-specific surface markers by flow cytometry,Results. Cultures with ChBM-derived CD34(+) cells showed the lowest Level of expansion of megakaryocytes and gave rise to more profound formation of myeloid and monocytic cells. In cultures with BM- or PB-derived cells, presence of IL-3 reduced the number of immature megakaryocytes (CD34(+)CD41(+) cells). However, in CB cultures, the number of CD34(+)CD41(+) cells was highest in cultures with Tpo + IL-3. Overall, cultures with CB CD34(+) cells yielded the highest number of megakaryocytes, but these cells showed reducedploidization and lower level of CD41 expression, suggesting less maturation. Conclusions. Each of the different CD34(+) cell sources responded differently to cytokine stimulation, For PB and ABM, the cytokine combination Tpo IL-I is most suitable to obtain high numbers of both immature and mature megakaryocytes for transfusion purposes. For CB, Tpo + IL-3 is better. (C) 2000 International Society for Experimental Hematology, Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:22:37