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Titolo:
MESSENGER-RNA STABILITY IS REGULATED BY A CODING-REGION ELEMENT AND THE UNIQUE 5' UNTRANSLATED LEADER SEQUENCES OF THE 3 SYNECHOCOCCUS-PSBATRANSCRIPTS
Autore:
KULKARNI RD; GOLDEN SS;
Indirizzi:
TEXAS A&M UNIV,DEPT BIOL COLLEGE STN TX 77843 TEXAS A&M UNIV,DEPT BIOL COLLEGE STN TX 77843
Titolo Testata:
Molecular microbiology
fascicolo: 6, volume: 24, anno: 1997,
pagine: 1131 - 1142
SICI:
0950-382X(1997)24:6<1131:MSIRBA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA STABILITY; HIGH LIGHT-INTENSITY; SP STRAIN PCC-7942; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; PHOTOSYSTEM-II; TRANSLATIONAL EFFICIENCY; SP STRAIN-PCC-7942; GENE-EXPRESSION; LOOP STRUCTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
R.D. Kulkarni e S.S. Golden, "MESSENGER-RNA STABILITY IS REGULATED BY A CODING-REGION ELEMENT AND THE UNIQUE 5' UNTRANSLATED LEADER SEQUENCES OF THE 3 SYNECHOCOCCUS-PSBATRANSCRIPTS", Molecular microbiology, 24(6), 1997, pp. 1131-1142

Abstract

The psbAl and psbAlll transcripts in Synechococcus sp. strain PCC 7942 are subject to accelerated turnover when cells are exposed to high light intensities, but psbAll message stability is unaffected. We used a psbAl 'minigene' which has a part of the coding sequence removed as a reporter gene in order to identify the cis-acting elements of the transcript that determine stability. While engineering the minigene to optimally mimic the native gene, we identified a stabilizer element within the open reading frame, corresponding to the coding region for thefirst membrane span of the D1 protein, the presence of and translation through which was essential for normal psbA mRNA stability. We propose that this stabilizer is a site for ribosome pausing, and that accumulation of ribosomes on the transcript upstream of the pause site increases stability. To identify the elements that regulate the differential responses of the psbA transcripts to high-light growth, sequences from psbAll and psbAlll were substituted in the psbAl minigene reporter. The chimeric reporter transcripts established that the psbAl and psbAlll untranslated leaders determine the faster turnover of these messages. The untranslated leader regions of the psbA transcripts may regulate mRNA stability by modulating translation and thereby stability, orby recruiting RNA-binding proteins that affect mRNA turnover more directly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 11:12:28