Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Selective involvement of interleukin-6 in the transcriptional activation of the suppressor of cytokine signaling-3 in the brain during systemic immune challenges
Autore:
Lebel, E; Vallieres, L; Rivest, S;
Indirizzi:
Univ Laval, CHUL, Res Ctr,Lab Mol Endocrinol, Dept Anat & Physiol, Quebec City, PQ G1V 4G2, Canada Univ Laval Quebec City PQ Canada G1V 4G2 Quebec City, PQ G1V 4G2, Canada
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 10, volume: 141, anno: 2000,
pagine: 3749 - 3763
SICI:
0013-7227(200010)141:10<3749:SIOIIT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICOTROPIN-RELEASING FACTOR; RAT-BRAIN; PROINFLAMMATORY CYTOKINE; INFLAMMATORY RESPONSE; NEGATIVE REGULATORS; LEPTIN RECEPTOR; NEURAL ACTIVITY; SOCS PROTEINS; KAPPA-B; TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Rivest, S Univ Laval, CHUL, Res Ctr,Lab Mol Endocrinol, Dept Anat & Physiol, 2705 Blvd Laurier, Quebec City, PQ G1V 4G2, Canada Univ Laval 2705 Blvd Laurier Quebec City PQ Canada G1V 4G2 anada
Citazione:
E. Lebel et al., "Selective involvement of interleukin-6 in the transcriptional activation of the suppressor of cytokine signaling-3 in the brain during systemic immune challenges", ENDOCRINOL, 141(10), 2000, pp. 3749-3763

Abstract

Cytokine-inducible proteins named as suppressors of cytokine signaling (SOCS) are rapidly induced by interleukin-6 (IL-6) and other members sharing the gp130 receptor subunit after activation of the Janus kinases (JAK) and the signal transducers and activators of transcription (STAT). These inhibitory proteins generally prevent tyrosine phosphorylation of IL-6 receptor signaling subunit gp130, specific JAK and STAT or in acting at steps distal to JAK activation. Expression of these inhibitory proteins is therefore a useful tool to investigate the signaling events occurring in the brain duringimmunogenic stimuli that involve cytokines of the IL-6 family. This study investigated the effect of ip lipopolysaccharide (LPS) administration on the expression of one key member of the SOCS family, SOCS-3, in both rats andmice. In rats, the endotoxin caused a profound transcriptional activation of the inhibitory factor in the circumventricular organs subfornical organ,organum vasculosum of the lamina terminalis, arcuate nucleus/median eminence, area postrema, choroid plexus, leptomeninges, ependymal lining cells, and along the endothelium of the brain blood vessels. The hybridization signal for SOCS-3 messenger RNA was low at 1 h, but robust at 3 and 6 h and declined to return to basal levels 12 h after the single ip LPS injection. Thepattern of SOCS-3 expression was similar in the brain of wildtype mice, although induction of the inhibitory factor was no longer observed in the ependymal lining cells of the cerebral ventricles and the blood microvessels of IL-6-deficient animals at an the times evaluated, i.e. from 1-8 h post-LPS injection. The endothelium of the brain capillaries also exhibited up-regulation of both IL-6 receptor and gp130 subunits during systemic inflammation, which allowed SOCS-3 expression in response to circulating IL-6. The present data indicate that the JAK/STAT transduction pathways that lead to SOCS-3 transcription are activated within cells accessible from the blood circulation, but not within deep parenchymal elements of the brain during endotoxemia. Induction of SOCS-3 followed the cascade of events that take placeduring the acute phase response and the contribution of IL-6 in activatingthe inhibitory factor is site specific and not generalized throughout the central nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:20:17