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Titolo:
Serrate and Notch specify cell fates in the heart field by suppressing cardiomyogenesis
Autore:
Rones, MS; McLaughlin, KA; Raffin, M; Mercola, M;
Indirizzi:
Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ed, Dept Cell Biol, Boston, MA 02115 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 17, volume: 127, anno: 2000,
pagine: 3865 - 3876
SICI:
0950-1991(200009)127:17<3865:SANSCF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE MORPHOGENETIC PROTEINS; XENOPUS-EMBRYOS; DROSOPHILA-NOTCH; DELTA-HOMOLOG; PRIMARY NEUROGENESIS; EARLY EMBRYOGENESIS; CARDIAC MYOGENESIS; MOUSE DEVELOPMENT; ALAGILLE-SYNDROME; VERTEBRATE HEART;
Keywords:
Notch; Serrate; heart; myocardium; mesocardium; Xenopus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
85
Recensione:
Indirizzi per estratti:
Indirizzo: Mercola, M Harvard Univ, Sch Med, Dept Cell Biol, 240 Longwood Ave, Boston, MA 02115 USA Harvard Univ 240 Longwood Ave Boston MA USA 02115 MA 02115 USA
Citazione:
M.S. Rones et al., "Serrate and Notch specify cell fates in the heart field by suppressing cardiomyogenesis", DEVELOPMENT, 127(17), 2000, pp. 3865-3876

Abstract

Notch signaling mediates numerous developmental cell fate decisions in organisms ranging from flies to humans, resulting in the generation of multiple cell types from equipotential precursors. In this paper, we present evidence that activation of Notch by its ligand Serrate apportions myogenic and non-myogenic cell fates within the early Xenopus heart field. The crescent-shaped field of heart mesoderm is specified initially as cardiomyogenic, While the ventral region of the field forms the myocardial tube, the dorsolateral portions lose myogenic potency and form the dorsal mesocardium and pericardial roof (Raffin, M., Leong, L. M., Rones, M. S., Sparrow, D., Mohun, T. and Mercola, M. (2000) Dev. Biol., 218, 326-340), The local interactionsthat establish or maintain the distinct myocardial and non-myocardial domains have never been described. Here we show that Xenopus Notch1 (Xotch) andSerrate1 are expressed in overlapping patterns in the early heart field. Conditional activation or inhibition of the Notch pathway with inducible dominant negative or active forms of the RBP-J/Suppressor of Hairless [Su(H)] transcription factor indicated that activation of Notch feeds back on Serrate1 gene expression to localize transcripts more dorsolaterally than those of Notch1, with overlap in the region of the developing mesocardium, Moreover, Notch pathway activation decreased myocardial gene expression and increased expression of a marker of the mesocardium and pericardial roof, whereas inhibition of Notch signaling had the opposite effect. Activation or inhibition of Notch also regulated contribution of individual cells to the myocardium. Importantly, expression of Nkx2.5 and Gata4 remained largely unaffected, indicating that Notch signaling functions downstream of heart field specification. We conclude that Notch signaling through Su(H) suppresses cardiomyogenesis and that this activity is essential for the correct specification of myocardial and non-myocardial cell fates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 23:04:38