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Titolo:
6-FluoroDOPA metabolism in rat striatum: time course of extracellular metabolites
Autore:
DeJesus, OT; Haaparanta, M; Solin, O; Nickles, RJ;
Indirizzi:
Univ Wisconsin, Sch Med, Dept Med Phys, Med Sci Ctr 1530, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 d Sci Ctr 1530, Madison, WI 53706 USA Turku PET Ctr, Turku, Finland Turku PET Ctr Turku FinlandTurku PET Ctr, Turku, Finland
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1, volume: 877, anno: 2000,
pagine: 31 - 36
SICI:
0006-8993(20000915)877:1<31:6MIRST>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON EMISSION TOMOGRAPHY; L-DOPA; 6-FLUORO-L-DOPA; MONKEYS; INVIVO;
Keywords:
FluoroDOPA; in vivo microdialysis; PET imaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: DeJesus, OT Univ Wisconsin, Sch Med, Dept Med Phys, Med Sci Ctr 1530, 1300Univ Ave, Madison, WI 53706 USA Univ Wisconsin 1300 Univ Ave Madison WI USA 53706 WI 53706 USA
Citazione:
O.T. DeJesus et al., "6-FluoroDOPA metabolism in rat striatum: time course of extracellular metabolites", BRAIN RES, 877(1), 2000, pp. 31-36

Abstract

6-[F-18]Fluoro-L-DOPA (FDOPA) is an imaging agent used in the study of dopamine terminals in the living brain using positron emission tomography (PET). To better understand the role of tracer metabolism in dynamic FDOPA PET studies, the pharmacokinetics of individual FDOPA metabolites in extracellular space in the striata of anesthetized rats was investigated using in vivo microdialysis. Brain tissues were also analysed to obtain FDOPA metabolite distribution in the combined intracellular and extracellular spaces. Total extracellular. [F-18] radioactivity in rat striata was observed to rise and peak at 30 min post-injection (p.i.) and declined with clearance half-life of 2 h. In the extracellular space, the dominant FDOPA metabolite at early times was FDOPAC, followed by FHVA at 50 min, then F-sulfoconjugates at 70 min and finally 3-O-methyl-6-Fluoro-L-DOPA (3OMFD) at later times. Theseresults are consistent with the sequential metabolism and brain clearance of L-DOPA and its metabolites. Analysis of whole striatal tissue confirmed the intraneuronal localization of fluorodopamine most likely stored in vesicles. A new but not unexpected finding was the enrichment of 3OMFD in intraneuronal striatal space which is perhaps a factor in its slow cerebral clearance. Since FDOPA PET data reflects the: overall pharmacokinetics of several [F-18]-metabolites, the observed different rates of formation and clearance and also different neuronal localization of each metabolite contribute to the measures obtained in dynamic FDOPA PET studies. These metabolic steps and their role in tracer kinetics are, thus, important factors to consider in ascribing physiologic significance to PET-derived measures. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 02/04/20 alle ore 17:41:30