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Titolo:
Hypoxia induces the expression of a 43-kDa protein (PROXY-1) in normal andmalignant cells
Autore:
Park, H; Adams, MA; Lachat, P; Bosman, F; Pang, SC; Graham, CH;
Indirizzi:
Queens Univ, Dept Anat & Cell Biol, Kingston, ON K7L 3N6, Canada Queens Univ Kingston ON Canada K7L 3N6 Biol, Kingston, ON K7L 3N6, Canada Queens Univ, Dept Pharmacol & Toxicol, Kingston, ON K7L 3N6, Canada QueensUniv Kingston ON Canada K7L 3N6 icol, Kingston, ON K7L 3N6, Canada Univ Lausanne, Dept Pathol, CH-1011 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1011 CH-1011 Lausanne, Switzerland
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 276, anno: 2000,
pagine: 321 - 328
SICI:
0006-291X(20000916)276:1<321:HITEOA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; HUMAN TROPHOBLAST CELLS; UROKINASE RECEPTOR EXPRESSION; DIFFERENTIAL DISPLAY ANALYSIS; MURINE TUMOR-CELLS; GENE-EXPRESSION; INDUCIBLE FACTOR-1; CARBON-MONOXIDE; HEME PROTEIN; OXYGEN;
Keywords:
hypoxia; oxygen; gene expression; protein; heme protein; mRNA; cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Graham, CH Queens Univ, Dept Anat & Cell Biol, Botterell Hall,9th Floor, Kingston, ONK7L 3N6, Canada Queens Univ Botterell Hall,9th Floor Kingston ONCanada K7L 3N6
Citazione:
H. Park et al., "Hypoxia induces the expression of a 43-kDa protein (PROXY-1) in normal andmalignant cells", BIOC BIOP R, 276(1), 2000, pp. 321-328

Abstract

This study was designed to determine the expression of cellular factors that may participate in phenotypic changes that occur under conditions of hypoxia. Using the RT-PCR differential display method, we isolated a cDNA fragment corresponding to a gene whose expression was induced in trophoblast and breast carcinoma cells cultured under 1 or 2% oxygen vs 4% oxygen or higher. This gene encodes a 43-kDa protein initially identified in homocysteine-treated endothelial cells and later shown to be upregulated in various human and mouse cell types (termed RTP, Drg1, Cap43, rit42, Ndr1), Herein we refer to this gene product as PROXY-1, for Protein Regulated by OXYgen-1. Elevated mRNA and protein levels were first observed ill cells cultured in 1%oxygen for 8 h, Although PROXY-1 mRNA levels returned to near-control values within 2 h of reexposure to 20% oxygen, protein levels remained high 72 h after reexposure to 20% oxygen. Treatment of cells with hypoxia mimics such as cobalt or iron chelators also increased PROXY-1 expression. Moreover,presence of 30% carbon monoxide in the hypoxic atmosphere abrogated the upregulation of PROXY-1 expression. These findings suggest that hypoxia upregulates PROXY-1 levels through a heme protein-dependent pathway and that assessment of PROXY-1 expression may be of potential use in evaluating tissue hypoxia, (C) 2000 Academic Press.

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Documento generato il 01/10/20 alle ore 07:40:29