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Titolo:
VEGF gene transfer mobilizes endothelial progenitor cells in patients withinoperable coronary disease
Autore:
Kalka, C; Tehrani, H; Laudenberg, B; Vale, PR; Isner, JM; Asahara, T; Symes, JF;
Indirizzi:
Tufts Univ, Sch Med, St Elizabeths Med Ctr, Div Cardiothorac Surg,Dept Vasc Med, Brighton, MA 02135 USA Tufts Univ Brighton MA USA 02135 rg,Dept Vasc Med, Brighton, MA 02135 USA Tufts Univ, Sch Med, St Elizabeths Med Ctr, Dept Cardiothorac Surg, Brighton, MA 02135 USA Tufts Univ Brighton MA USA 02135 ardiothorac Surg, Brighton, MA 02135 USA
Titolo Testata:
ANNALS OF THORACIC SURGERY
fascicolo: 3, volume: 70, anno: 2000,
pagine: 829 - 834
SICI:
0003-4975(200009)70:3<829:VGTMEP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLATERAL VESSEL DEVELOPMENT; GROWTH-FACTOR; COMMON PRECURSOR; ANGIOGENESIS; NEOVASCULARIZATION; EXPRESSION; VASCULOGENESIS; IMPAIRMENT; ISCHEMIA; THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Symes, JF Tufts Univ, Sch Med, St Elizabeths Med Ctr, Div Cardiothorac Surg,Dept Vasc Med, 11 Nevins St,MOB 306, Brighton, MA 02135 USA Tufts Univ 11Nevins St,MOB 306 Brighton MA USA 02135 02135 USA
Citazione:
C. Kalka et al., "VEGF gene transfer mobilizes endothelial progenitor cells in patients withinoperable coronary disease", ANN THORAC, 70(3), 2000, pp. 829-834

Abstract

Background. Direct transfection of ischemic myocardium with naked plasmid DNA encoding for vascular endothelial growth factor-165 (VEGF(165)) has been shown to mobilize endothelial progenitor cells (EPCs). This study examined the kinetics of circulating EPCs isolated from peripheral blood mononuclear cells after gene transfer, and their role in neovascularization of ischemic myocardium. Methods. The mononuclear cell population was isolated from peripheral venous blood samples of patients with functional class III or IV angina receiving intramyocardial VEGF(165) gene transfer. Peripheral blood mononuclear cells were examined by an in vitro EPC culture assay and fluorescent-activatecell sorting. The data were compared with a control group consisting of patients who had undergone off-pump coronary artery bypass grafting without receiving gene transfer. Results. Coinciding with a rise in VEGF levels, mobilization of EPCs increased significantly over base line for 9 weeks after the treatment (121 +/- 14 cells/mm(2) versus 36.8 +/- 8 cells/mm(2), p < 0.0005), followed by a subsequent decrease. Fluorescent-activated cell sorting analysis confirmed culture assay data, with a statistically significant rise in cells expressingvascular endothelial-cadherin, CD51/61 [alpha(v)beta(3)], CD62E [E-selectin], CD34, and KDR. The control group failed to show significant mobilization of EPCs. Conclusions. Mobilization of EPCs with resultant postnatal vasculogenesis,may play a role in revascularizing ischemic myocardium following human gene transfer with VEGF(165). (Ann Thorac Surg 2000;70:829-34) (C) 2000 by TheSociety of Thoracic Surgeons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:28:37