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Titolo:
Evaluation of the binding between potential anti-HIV DNA-based drugs and viral envelope glycoprotein gp120 by capillary electrophoresis with laser-induced fluorescence detection
Autore:
Zhou, W; Tomer, KB; Khaledi, MG;
Indirizzi:
N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 pt Chem, Raleigh, NC 27695 USA NIEHS, Struct Biol Lab, NIH, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 ab, NIH, Res Triangle Pk, NC 27709 USA
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 2, volume: 284, anno: 2000,
pagine: 334 - 341
SICI:
0003-2697(20000910)284:2<334:EOTBBP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; LOOP DOMAIN V3; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES; DEXTRAN SULFATE; AFFINITY; PROTEIN; RECEPTOR; CD4; INFECTION; IDENTIFICATION;
Keywords:
phosphorothioate oligodeoxynucleotide; HIV envelope glycoprotein gp120; affinity-capillary electrophoresis; laser-induced fluorescence detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Khaledi, MG N Carolina State Univ, Dept Chem, Box 8204, Raleigh, NC 27695 USA N Carolina State Univ Box 8204 Raleigh NC USA 27695 27695 USA
Citazione:
W. Zhou et al., "Evaluation of the binding between potential anti-HIV DNA-based drugs and viral envelope glycoprotein gp120 by capillary electrophoresis with laser-induced fluorescence detection", ANALYT BIOC, 284(2), 2000, pp. 334-341

Abstract

The fusion of the human immunodeficiency virus (HIV) with the target cell was assisted by the interaction between the viral envelope glycoprotein HIV-1 gp120 and a chemokine receptor. Studies have shown that the efficiency of the binding depends on the presence of the V3 loop of the gp120 which is known to interact with polyanions, such as phosphorothioate oligodeoxynucleotides (Sd, potential anti-HIV drugs). In this study, capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was used to systematically evaluate binding between Sd and HIV-1 gp120. A 25-mer fluorescentlytagged phosphorothioate oligodeoxynucleotide (GEM) was employed as a probeto study this interaction. The dissociation constant (K-d) between GEM andgp120 was determined to be 0.98 nM by Scatchard analysis. The competition constants (K-c) of a set of Sd that compete with GEM for binding to gp120 were also determined. The results showed that the interaction had a strong dependence on the sulfur phosphorothioate backbone. Chain length and the sequence of Sd also affect the ability of binding to gp120. The ability to study the protein-drug binding in the solution with minimal sample consumptionmakes CE-LIF very attractive for biological studies. (C) 2000 Academic Press.

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Documento generato il 29/09/20 alle ore 10:27:49