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Titolo:
Single-step gas chromatography-mass spectrometry analysis of glycolipid constituents as heptafluorobutyrate derivatives with a special reference to the lipid portion
Autore:
Pons, A; Popa, J; Portoukalian, J; Bodennec, J; Ardail, D; Kol, O; Martin-Martin, MJ; Hueso, P; Timmerman, P; Leroy, Y; Zanetta, JP;
Indirizzi:
Univ Sci & Tech Lille Flandres Artois, Chim Biol Lab, CNRS, UMR 8576, F-59655 Villeneuve Dascq, France Univ Sci & Tech Lille Flandres Artois Villeneuve Dascq France F-59655 ce Univ Lyon 1, Lab Glycobiol, Fac Med, F-69921 Oullins, France Univ Lyon 1 Oullins France F-69921 iol, Fac Med, F-69921 Oullins, France Fac Med Lyon Sud, INSERM, U189, Lyon, France Fac Med Lyon Sud Lyon France c Med Lyon Sud, INSERM, U189, Lyon, France Univ Salamanca, Fac Biol, Dept Bioquim & Biol Mol, E-37008 Salamanca, Spain Univ Salamanca Salamanca Spain E-37008 iol Mol, E-37008 Salamanca, Spain
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 2, volume: 284, anno: 2000,
pagine: 201 - 216
SICI:
0003-2697(20000910)284:2<201:SGCSAO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
O-METHYL-GLYCOSIDES; LIQUID-CHROMATOGRAPHY; PLASMA-MEMBRANES; SIALIC ACIDS; RAT-BRAIN; OLIGOSACCHARIDES; SPHINGOLIPIDS; GLYCOPROTEINS; GANGLIOSIDES; EXTRACTION;
Keywords:
gas chromatography; mass spectrometry; ceramide; ganglioside; dimethyl acetal; long-chain base; sphingoid base; alkylacylglycerol; fatty acid; monosaccharide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Zanetta, JP Univ Sci & Tech Lille Flandres Artois, Chim Biol Lab, CNRS, UMR 8576, F-59655 Villeneuve Dascq, France Univ Sci & Tech Lille Flandres Artois Villeneuve Dascq France F-59655
Citazione:
A. Pons et al., "Single-step gas chromatography-mass spectrometry analysis of glycolipid constituents as heptafluorobutyrate derivatives with a special reference to the lipid portion", ANALYT BIOC, 284(2), 2000, pp. 201-216

Abstract

In a previous work (Zanetta et al. Glycobiology 9, 255-266 (1999)), it wasreported that all constituents of gangliosides could be obtained as heptafluorobutyrate derivatives after methanolysis in a single gas chromatographyanalysis. This report demonstrates that gas chromatography coupled with mass spectrometry in the electron impact mode allows identification and quantification of long-chain bases and fatty acids without interference from monosaccharides. On the basis of ions specific for families and for individualcompounds, sphingosines, sphinganines, and phytosphingosines (including ramified, unsaturated, hydroxylated, and etherified compounds) can be identified. Fatty acid methyl esters, including linear, ramified, unsaturated, andhydroxylated species, are identified and quantified in the same way. Possible extensions of this method to the fatty moiety of other lipids (alkylacylglycerol and dimethyl acetal) are discussed. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:38:48