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Titolo:
Localization of organic cation transporters OCT1 and OCT2 in rat kidney
Autore:
Karbach, U; Kricke, J; Meyer-Wentrup, F; Gorboulev, V; Volk, C; Loffing-Cueni, D; Kaissling, B; Bachmann, S; Koepsell, H;
Indirizzi:
Charite, Inst Anat, D-10098 Berlin, Germany Charite Berlin Germany D-10098 arite, Inst Anat, D-10098 Berlin, Germany Inst Anat, CH-8051 Zurich, Switzerland Inst Anat Zurich Switzerland CH-8051 t Anat, CH-8051 Zurich, Switzerland
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 4, volume: 279, anno: 2000,
pagine: F679 - F687
SICI:
0363-6127(200010)279:4<F679:LOOCTO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL EXPRESSION; SUBSTRATE-SPECIFICITY; MEMBRANE LOCALIZATION; ANTIGEN RETRIEVAL; TISSUE-SECTIONS; IN-SITU; CLONING; CELLS; ROCT1; VESICLES;
Keywords:
polyspecific cation transporter; organic cation transporter; family; gene expression; kidney; immunohistochemisty; in; situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Koepsell, H Univ Wurzburg, Inst Anat, Koellikerstr 6, D-97070 Wurzburg, Germany Univ Wurzburg Koellikerstr 6 Wurzburg Germany D-97070 Germany
Citazione:
U. Karbach et al., "Localization of organic cation transporters OCT1 and OCT2 in rat kidney", AM J P-REN, 279(4), 2000, pp. F679-F687

Abstract

Renal excretion and reabsorption of organic cations are mediated by electrogenic and electroneutral organic cation transporters, which belong to a recently discovered family of polyspecific transporters. These transporters are electrogenic and exhibit differences in substrate specificity. In rat, the renal expression of the polyspecific cation transporters rOCT1 and rOCT2was investigated. By in situ hybridization, significant amounts of both rOCT1 and rOCT2 mRNA were detected in S1, S2, and S3 segments of proximal tubules. By immunohistochemistry, expression of the rOCT1 protein was mainly observed in S1 and S2 segments of proximal tubules, with lower expression levels in the S3 segments. At variance, rOCT2 protein was mainly expressed inthe S2 and S3 segments. Both transporters were localized to the basolateral cell membrane. Neither rOCT1 nor rOCT2 was detected in the vasculature, the glomeruli, and nephron segments other than proximal tubules. The data suggest that rOCT1 and rOCT2 are responsible for basolateral cation uptake inthe proximal tubule, which represents the first step in cation secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 04:50:35