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Titolo:
Identification of a major B-cell epitope of the Plasmodium falciparum glutamate-rich protein (GLURP), targeted by human antibodies mediating parasitekilling
Autore:
Theisen, M; Soe, S; Jessing, SG; Okkels, LM; Danielsen, S; Oeuvray, C; Druilhe, P; Jepsen, S;
Indirizzi:
Statens Serum Inst, Dept Clin Biochem, Copenhagen, Denmark Statens Serum Inst Copenhagen Denmark Clin Biochem, Copenhagen, Denmark Dept Med Res, Yangon, Myanmar Dept Med Res Yangon MyanmarDept Med Res, Yangon, Myanmar Inst Pasteur, Lab Parasitol Med, Paris, France Inst Pasteur Paris France nst Pasteur, Lab Parasitol Med, Paris, France
Titolo Testata:
VACCINE
fascicolo: 2-3, volume: 19, anno: 2000,
pagine: 204 - 212
SICI:
0264-410X(20000915)19:2-3<204:IOAMBE>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASEXUAL BLOOD STAGES; MALARIA VACCINE; IMMUNOGLOBULIN-M; MONOCYTES; SPF66; MECHANISMS; CHILDREN; INVITRO; GROWTH; TRIAL;
Keywords:
Plasmodium falciparum; glutamate-rich protein; B-cell epitope; antibody-dependent cellular inhibition; phase-display;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Theisen, M Statens Serum Inst, Dept Clin Biochem, Copenhagen, Denmark Statens Serum Inst Copenhagen Denmark m, Copenhagen, Denmark
Citazione:
M. Theisen et al., "Identification of a major B-cell epitope of the Plasmodium falciparum glutamate-rich protein (GLURP), targeted by human antibodies mediating parasitekilling", VACCINE, 19(2-3), 2000, pp. 204-212

Abstract

The antigenicity of the glutamate-rich protein (GLURP) of Plasmodium falciparum was comprehensively evaluated in epitope-mapping studies utilizing a phage display library, synthetic peptides and anti-GLURP IgG preparations previously shown to promote strong antibody-dependent cellular inhibition (ADCI) effects. We identified six major B-cell epitopes within the nonrepetitive region RO, corresponding to amino acid residues 173 to 187 (P1), 193 to207 (P3), 216 to 229 (P4), 264 to 288 (P11), 343 to 357 (P10), and 407 to 434 (S3). Of these, four (P1, P3, P4, and S3) were frequently recognized byhigh-titered Ige antibodies in plasma samples from immune Liberian adults (prevalence: 29.1-45.0%). The three epitopes P1, P3, and P4 contained a common motif (seven out of nine positions are identical) and may thus constitute a family of structurally related epitopes. This leaves two distinct epitopes, one (P3) representing this new epitope family and S3 as targets for biologically active antibodies. Human IgG antibodies from single plasma samples were affinity-purified against these peptides. P3-specific IgG preparations were consistently more effective in ADCI than S3-specific Igc. Among the different GLURP epitopes, we therefore suggest that the P3 epitope is potentially the most important epitope in GLURP for the development of clinical immunity to malaria in man. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:34:08