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Titolo:
Refolding of rRNA exons enhances dissociation of the Tetrahymena intron
Autore:
Cao, Y; Woodson, SA;
Indirizzi:
Univ Maryland, Cell & Mol Biol Program, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 l Program, College Pk, MD 20742 USA Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 & Biochem, College Pk, MD 20742 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 9, volume: 6, anno: 2000,
pagine: 1248 - 1256
SICI:
1355-8382(200009)6:9<1248:ROREED>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; SELF-SPLICING RNA; PRE-RIBOSOMAL-RNA; ACTIVE-SITE; THERMOPHILA RIBOZYME; KINETIC DESCRIPTION; SECONDARY STRUCTURE; BASE-PAIRS; SUBSTRATE; CATALYSIS;
Keywords:
group I intron; product dissociation; ribozyme; rRNA; self-splicing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Woodson, SA Johns Hopkins Univ, Dept Biophys, Baltimore, MD 21218 USA Johns Hopkins Univ Baltimore MD USA 21218 imore, MD 21218 USA
Citazione:
Y. Cao e S.A. Woodson, "Refolding of rRNA exons enhances dissociation of the Tetrahymena intron", RNA, 6(9), 2000, pp. 1248-1256

Abstract

Self-splicing of the Tetrahymena pre-rRNA is inhibited by a conserved rRNAhairpin P(-1) upstream of the 5' splice site. P(-1) inhibits self-splicingby competing with formation of the P1 splice site helix. Here we show thatthe P(-1) hairpin also enhances dissociation of the spliced products, which was monitored by native gel electrophoresis. Mutations that stabilize therRNA hairpin increase the rate of dissociation approximately 10-fold, from0.5 min(-1) for the wild-type RNA to similar to 4 min(-1) at 30 degrees C. Conversely, mutations or oligonucleotides that inhibit refolding of the exons and that stabilize the P1 helix decrease the rate of product release. The results suggest that refolding of products can be used to stimulate the turnover of ribozyme-catalyzed reactions. In the pre-rRNA, this conformational change helps shift the equilibrium of self-splicing toward the mature rRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 11:46:09