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Titolo:
Anterograde flow of cargo across the Golgi stack potentially mediated via bidirectional "percolating" COPI vesicles
Autore:
Orci, L; Ravazzola, M; Volchuk, A; Engel, T; Gmachl, M; Amherdt, M; Perrelet, A; Sollner, TH; Rothman, JE;
Indirizzi:
Univ Geneva, Sch Med, Dept Morphol, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 Morphol, CH-1211 Geneva 4, Switzerland
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 19, volume: 97, anno: 2000,
pagine: 10400 - 10405
SICI:
0027-8424(20000912)97:19<10400:AFOCAT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM; VESICULAR TRANSPORT; CISTERNAL MATURATION; PROTEIN-TRANSPORT; MAMMALIAN-CELLS; MEMBRANE-FUSION; MATRIX PROTEIN; BULK FLOW; COMPLEX; SNARE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Rothman, JE Mem Sloan Kettering Canc Ctr, Cellular Biochem & Biophys Program, 1275 York Ave,Box 251, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr 1275 York Ave,Box 251 New York NY USA 10021
Citazione:
L. Orci et al., "Anterograde flow of cargo across the Golgi stack potentially mediated via bidirectional "percolating" COPI vesicles", P NAS US, 97(19), 2000, pp. 10400-10405

Abstract

How do secretory proteins and other cargo targeted to post-Golgi locationstraverse the Golgi stack? We report immunoelectron microscopy experiments establishing that a Golgi-restricted SNARE, GOS 28, is present in the same population of COPI vesicles as anterograde cargo marked by vesicular stomatitis virus glycoprotein, but is excluded from the COPI vesicles containing retrograde-targeted cargo (marked by KDEL receptor), We also report that GOS 28 and its partnering t-SNARE heavy chain, syntaxin 5, reside together inevery cisterna of the stack. Taken together, these data raise the possibility that the anterograde cargo-laden COPI vesicles, retained locally by means of tethers, are inherently capable of fusing with neighboring cisternae on either side. If so, quanta of exported proteins would transit the stack in COS 28-COPI vesicles via a bidirectional random walk, entering at the cis face and leaving at the trans face and percolating up and down the stack in between. Percolating vesicles carrying both post-Golgi cargo and Golgi residents up and down the stack would reconcile disparate observations on Golgi transport in cells and in cell-free systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 03:35:09