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Titolo:
Glutathione S-transferase does not play a role in multidrug resistance of L1210/VCR cell line
Autore:
Bohacova, V; Kvackajova, J; Barancik, M; Drobna, Z; Breier, A;
Indirizzi:
Slovak Acad Sci, Inst Mol Physiol & Genet, Bratislava 83334, Slovakia Slovak Acad Sci Bratislava Slovakia 83334 et, Bratislava 83334, Slovakia Slovak Acad Sci, Heart Res Inst, Bratislava 83334, Slovakia Slovak Acad Sci Bratislava Slovakia 83334 st, Bratislava 83334, Slovakia
Titolo Testata:
PHYSIOLOGICAL RESEARCH
fascicolo: 4, volume: 49, anno: 2000,
pagine: 447 - 453
SICI:
0862-8408(2000)49:4<447:GSDNPA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED VINCRISTINE RESISTANCE; MOUSE LEUKEMIC-CELLS; P-GLYCOPROTEIN; TOPOISOMERASE-II; FIBROSARCOMA CELLS; PLASMA-MEMBRANE; MITOMYCIN-C; K562 CELLS; OVEREXPRESSION; EXPRESSION;
Keywords:
P-glycoprotein; multidrug resistance; L1210 leukemic cell lines; cytostatic drugs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Breier, A Slovak Acad Sci, Inst Mol Physiol & Genet, Vlarska 5, Bratislava83334, Slovakia Slovak Acad Sci Vlarska 5 Bratislava Slovakia 83334 4, Slovakia
Citazione:
V. Bohacova et al., "Glutathione S-transferase does not play a role in multidrug resistance of L1210/VCR cell line", PHYSL RES, 49(4), 2000, pp. 447-453

Abstract

Multidrug resistance of cancer cells is often accompanied by the (over)expression of integral plasma membrane P-glycoprotein, an ATP-dependent transport pump for diverse unrelated compounds. The glutathione detoxification system represents another mechanism that may be involved in multidrug resistance. In the multidrug-resistant L1210/VCR cell fine obtained by long-term adaptation of parental L1210 cells to vincristine, an increased expression of P-glycoprotein has previously been established. In this paper, we investigated if the glutathione detoxification system is also involved in the multidrug resistance of these cells. L1210/VCR cells with resistance induced byadaptation to vincristine were also found to be cross-resistant to vinblastine, actinomycin D, mitomycin C, doxorubicin and cyclophosphamide. The resistance of the above cells to vincristine and doxorubicin was accompanied by a depression of drug accumulation (which has not yet been established forother drug). L1210/VCR cells are able to survive better than sensitive cells under conditions when glutathione was depleted by L-buthionine sulfoximine. Nevertheless, L-buthionine sulfoximine did not influence the resistanceof L1210/VCR cells to vincristine. Moreover, the presence of sublethal concentrations of cytostatics neither changed the ICS, value of resistant cells to L-buthionine sulfoximine nor the cytoplasmic activity of glutathione S-transferase, the crucial enzyme of glutathione detoxification system. All the above findings indicate that the glutathione detoxification system is not involved in the mechanisms that ensure the multidrug resistance phenotype of L1210/VCR cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:32:32