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Titolo:
P2x(2) receptor immunoreactivity in the dorsal vagal complex and area postrema of the rat
Autore:
Atkinson, L; Batten, TFC; Deuchars, J;
Indirizzi:
Univ Leeds, Sch Biomed Sci, Leeds LS2 9NQ, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9NQ S2 9NQ, W Yorkshire, England Univ Leeds, Inst Cardiovasc Res, Leeds LS2 9NQ, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9NQ S2 9NQ, W Yorkshire, England
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 99, anno: 2000,
pagine: 683 - 696
SICI:
0306-4522(2000)99:4<683:PRIITD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEUS-TRACTUS-SOLITARIUS; GATED ION CHANNELS; FAST SYNAPTIC TRANSMISSION; CENTRAL-NERVOUS-SYSTEM; SENSORY NEURONS; ATP RECEPTOR; PHARMACOLOGICAL CHARACTERIZATION; GLUTAMATE-IMMUNOREACTIVITY; HORSERADISH-PEROXIDASE; EXTRACELLULAR ATP;
Keywords:
P2X(2) receptor; nucleus tractus solitarius; vagal afferent fibres; dorsal vagal motor nucleus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Deuchars, J Univ Leeds, Sch Biomed Sci, Leeds LS2 9NQ, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9NQ rkshire, England
Citazione:
L. Atkinson et al., "P2x(2) receptor immunoreactivity in the dorsal vagal complex and area postrema of the rat", NEUROSCIENC, 99(4), 2000, pp. 683-696

Abstract

Adenosine 5'-triphosphate (ATP) can function as a fast synaptic transmitter through its actions on ionotropic (P2X) and metabotropic (P2Y) receptors in neuronal tissue. The ionotropic receptors have been classified into seven subtypes (P2X(1)-P2X(7)) by molecular cloning. However, they are difficult to distinguish pharmacologically owing to an absence of specific agonistsand antagonists. In this study we used neuroanatomical methods to determine the origin and neurochemical phenotype of the P2X(2) subtype of purinoceptor in the dorsal medulla of the rat. Using immunohistochemistry we observed dense networks of P2X(2) receptor immunoreactive labelled fibres and terminals in the dorsal vagal complex and area postrema, as well as labelled cell bodies in the dorsal vagal nucleus and the area postrema. The P2X(2) receptor was localized presynaptically in vagal afferent fibres and terminals in the nucleus tractus solitarius at the ultrastructural level by combininginjections of an anterograde tracer (biotin dextran amine) into the nodoseganglion with pre-embedding immunogold visualization of P2X(2) immunoreactivity. Terminals immunoreactive for the P2X(2) receptor in the nucleus tractus solitarius were found to contain glutamate, but not GABA immunoreactivity by post-embedding immunogold-labelling techniques. In cell bodies in thearea postrema, dual immunofluorescence also indicated that P2X(2) receptorimmunoreactive cells are glutamatergic but not GABAergic. The P2X(2) receptor was localized to vagal preganglionic neurons in the dorsal vagal nucleus that were identified by prior intraperitoneal injections of the retrograde tracer FluoroGold. No cells immunoreactive for the P2X(2) receptor were observed in the nucleus tractus solitarius. The localization of P2X(2) receptor immunoreactivity presynaptically in vagal afferent terminals indicates that the receptor may be involved in modulating transmitter release from vagal afferent fibres. Furthermore, the presence of the P2X(2) receptor in vagal preganglionic cells and in glutamatergic cells of the area postrema implies that it may, respectively, play a role in regulation of vagal efferent cell activity and modulation of excitatory outputs from the area postrema to other brain regions. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:37:51