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Titolo:
Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae
Autore:
Terashima, H; Yabuki, N; Arisawa, M; Hamada, K; Kitada, K;
Indirizzi:
Nippon Roche Res Ctr, Dept Mycol, Kanagawa 2478530, Japan Nippon Roche ResCtr Kanagawa Japan 2478530 col, Kanagawa 2478530, Japan
Titolo Testata:
MOLECULAR AND GENERAL GENETICS
fascicolo: 1-2, volume: 264, anno: 2000,
pagine: 64 - 74
SICI:
0026-8925(200009)264:1-2<64:UOGEG(>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAP KINASE PATHWAY; 1,3-BETA-D-GLUCAN SYNTHASE; CHITIN SYNTHESIS; PLASMA-MEMBRANE; CROSS-LINKING; BETA-1,3-GLUCAN CONTENT; SEPTUM FORMATION; S-CEREVISIAE; YEAST; INTEGRITY;
Keywords:
FKS1; cell wall damage; RLM1; GPI proteins; Saccharomyces cerevisiae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Kitada, K Nippon Roche Res Ctr, Dept Mycol, 200 Kajiwara, Kanagawa 2478530, Japan Nippon Roche Res Ctr 200 Kajiwara Kanagawa Japan 2478530 Japan
Citazione:
H. Terashima et al., "Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae", MOL G GENET, 264(1-2), 2000, pp. 64-74

Abstract

FKS1 and FKS2 encode alternative catalytic subunits of the glucan synthases that are responsible for synthesis of beta-1,3-glucan in the Saccharomyces cerevisiae cell wall. Disruption of FKS1 reduces the glucan content of the cell wall. increases chitin content and activates the expression of CWP1,which encodes a glycosylphosphatidylinositol (GPI)-dependent cell wall protein. These cellular responses have been regarded as compensating for cell wall damage in order to maintain cell wall integrity. Here. we report the identification, by genome-wide screening, of 22 genes that are transcriptionally up-regulated in fks1 Delta cells. Among them, five genes were found toencode GPI-attached proteins, three of which are covalently associated with the cell wall. Deletion and replacement analysis of the promoter regions identified Rlm1-binding sequences as being responsible for the up-regulation following disruption of FKS1. Using the rlm1 Delta tetOp-FKS1 strain, in which the expression of FKS1 can be repressed by doxycycline, we examined the requirement for Rlm1 for the transcriptional up-regulation of these fivegenes. Three of the five genes were not up-regulated by doxycycline, indicating that Rlm1 mediates their up-regulation when FKS1 is inactivated. The remaining two genes were up-regulated by doxycycline, suggesting that a transcription factor other than Rlm1 is involved in their response to disruption of FKS1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 12:54:36