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Titolo:
Molecular ratio between borna disease viral-p40 and-p24 proteins in infected cells determined by quantitative antigen capture ELISA
Autore:
Watanabe, M; Zhong, Q; Kobayashi, T; Kamitani, W; Tomonaga, K; Ikuta, K;
Indirizzi:
Osaka Univ, Microbial Dis Res Inst, Dept Virol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 t Virol, Suita, Osaka 5650871, Japan Hokkaido Univ, Inst Immunol Sci, Sect Serol, Sapporo, Hokkaido 0600815, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600815 oro, Hokkaido 0600815, Japan
Titolo Testata:
MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 9, volume: 44, anno: 2000,
pagine: 765 - 772
SICI:
0385-5600(2000)44:9<765:MRBBDV>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; VESICULAR STOMATITIS-VIRUS; P-PROTEIN; MONOCLONAL-ANTIBODIES; DETECTING ANTIBODIES; NUCLEAR-LOCALIZATION; GENOME REPLICATION; STAGGERING DISEASE; SEQUENCE; NUCLEOPROTEIN;
Keywords:
borna disease virus; antigen capture ELISA; quantitative; p40 and p24 proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Tomonaga, K Osaka Univ, Microbial Dis Res Inst, Dept Virol, 3-1 Yamadaoka,Suita, Osaka 5650871, Japan Osaka Univ 3-1 Yamadaoka Suita Osaka Japan 5650871 0871, Japan
Citazione:
M. Watanabe et al., "Molecular ratio between borna disease viral-p40 and-p24 proteins in infected cells determined by quantitative antigen capture ELISA", MICROB IMMU, 44(9), 2000, pp. 765-772

Abstract

We developed the antigen capture enzyme-linked immunosorbent assay (ELISA)systems for quantification of Borna disease virus (BDV) major antigens, p40 and p24, Using these ELISAs, we quantified the two proteins in various BDV-infected materials, including the cell lysates and culture supernatants as well as the homogenates of experimental animal brains. The ELISAs were also applied to measure the infectious titer of BDV in persistently infected cell lines. Quantitative analysis with these ELISAs allowed us to measure the molecular ratio between the p40 and p24 in infected samples, Interestingly, the ratio of p24 to p40 in persistently infected cells was much higher than that observed in acutely infected cells although the ratios in the supernatants from both cell lines were quite similar, BDV-inoculated gerbil brain cells showed a relatively high ratio of p24 to p40 as compared with acutely infected cells. These observations suggested that the molecular ratio between the proteins strongly depended on the infectious status of BDV in the host cells, The ELISA system developed here could be a convenient methodfor the quantification of BDV infection and may also be beneficial for understanding viral replication and infectious status in the host cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 21:48:15