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Titolo:
Hypertonicity-induced intracellular pH changes in rat mast cells
Autore:
Cabado, AG; Alfonso, A; Vieytes, MR; Botana, LM;
Indirizzi:
Univ Santiago Compostela, Fac Vet, Dept Farmacol, E-27002 Lugo, Spain UnivSantiago Compostela Lugo Spain E-27002 armacol, E-27002 Lugo, Spain Univ Santiago Compostela, Fac Vet, Dept Fisiol, E-27002 Lugo, Spain Univ Santiago Compostela Lugo Spain E-27002 Fisiol, E-27002 Lugo, Spain
Titolo Testata:
LIFE SCIENCES
fascicolo: 16, volume: 67, anno: 2000,
pagine: 1969 - 1982
SICI:
0024-3205(20000908)67:16<1969:HIPCIR>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+-H+ EXCHANGER; PROTEIN-KINASE; HISTAMINE-RELEASE; VOLUME REGULATION; FUNCTIONAL-CHARACTERIZATION; OSMOTIC ACTIVATION; HUMAN-PLATELETS; CYTOPLASMIC PH; ATP DEPENDENCE; LYMPHOCYTES;
Keywords:
pHi; hypertonicity; mast cells; Na+/H+ exchanger; anion exchanger;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Botana, LM Univ Santiago Compostela, Fac Vet, Dept Farmacol, E-27002 Lugo,Spain Univ Santiago Compostela Lugo Spain E-27002 27002 Lugo, Spain
Citazione:
A.G. Cabado et al., "Hypertonicity-induced intracellular pH changes in rat mast cells", LIFE SCI, 67(16), 2000, pp. 1969-1982

Abstract

In a non-isotonic environment, cells can shrink or swell and return to their normal shape by activating ion transport pathways. Changes in intracellular pH (pHi) after osmotic stress have been identified in several cells. Inorder to study the mechanisms that regulate cytosolic pH of rat mast cellsin a hypertonic medium, we used the pH sensitive dye, BCECF. Under these hypertonic conditions, pHi undergoes an alkalinization following an initial acidification. The alkalinization is mediated by a Na+/H+ exchanger, since it is inhibited by amiloride and lack of extracellular sodium. Under these conditions, the alkalinization is increased with the PKC activators, TPA and GAG, and partially blocked with trifluoperazine, an unspecific protein kinase C (PKC) and Ca+2 calmodulin-dependent protein kinases (Ca+2/CaM Ii) inhibitor. There is also an anion exchanger, blocked with DIDS but not activated by PKC, that participates in the observed alkalinization. However, Na+/M+ exchanger is the main mechanism involved in the alkalinization of pHi ofmast cells in a hyperosmotic environment. (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:21:04