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Titolo:
Neuroinvasion by human respiratory coronaviruses
Autore:
Arbour, N; Day, R; Newcombe, J; Talbot, PJ;
Indirizzi:
Univ Quebec, INRS Inst Armand Frappier, Human Hlth Res Ctr, Lab Neuroimmunol, Laval, PQ H7V 1B7, Canada Univ Quebec Laval PQ Canada H7V 1B7 uroimmunol, Laval, PQ H7V 1B7, Canada Univ Sherbrooke, Dept Pharmacol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 erbrooke, PQ J1H 5N4, Canada Univ Coll London, Inst Neurol, London WC1N 1PJ, England Univ Coll London London England WC1N 1PJ eurol, London WC1N 1PJ, England
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 19, volume: 74, anno: 2000,
pagine: 8913 - 8921
SICI:
0022-538X(200010)74:19<8913:NBHRC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MOUSE HEPATITIS-VIRUS; MULTIPLE-SCLEROSIS PATIENTS; MYELIN BASIC-PROTEIN; PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY; INDUCED DEMYELINATING DISEASE; PERSISTENT INFECTION; MOLECULAR-BIOLOGY; SEQUENCE-ANALYSIS; VIRAL-INFECTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Talbot, PJ Univ Quebec, INRS Inst Armand Frappier, Human Hlth Res Ctr, LabNeuroimmunol, 531 Blvd Prairies, Laval, PQ H7V 1B7, Canada Univ Quebec 531Blvd Prairies Laval PQ Canada H7V 1B7 7, Canada
Citazione:
N. Arbour et al., "Neuroinvasion by human respiratory coronaviruses", J VIROLOGY, 74(19), 2000, pp. 8913-8921

Abstract

Human coronaviruses (HCoV) cause common colds but can also infect neural cell cultures. To provide definitive experimental evidence for the neurotropism and neuroinvasion of HCoV and its possible association with multiple sclerosis (MS), we have performed an extensive search and characterization ofHCoV RNA in a large panel of human brain autopsy samples. Very stringent reverse transcription-PCR,vith two primer pairs for both viral strains (229Eand OC43), combined with Southern hybridization, was performed on samples from 90 coded donors with various neurological diseases (39 with MS and 26 with other neurological diseases) or normal controls (25 patients). We report that 34% (40 of 90) of donors mere positive for 229E and that 23% (21 of90) were positive for OC43. A statistically significant higher prevalence of OC43 in MS patients (35.9%; 14 of 39) than in controls (13.7%; 7 of 51) was observed. Sequencing of nucleocapsid protein (N) gene amplicons revealed point mutations in OC43, some consistently found in three MS patient brains and one normal control but never observed in laboratory viruses. In situhybridization confirmed the presence of viral RNA in brain parenchyma, outside blood vessels. The presence of HCoV in human brains is consistent withneuroinvasion by these respiratory pathogens. Further studies are needed to distinguish between opportunistic and disease-associated viral presence in human brains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 13:43:14