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Titolo:
Gz can mediate the acute actions of mu- and kappa-opioids but is not involved in opioid-induced adenylyl cyclase supersensitization
Autore:
Tso, PH; Wong, YH;
Indirizzi:
Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol Kowloon Hong Kong Peoples R China s R China Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Kowloon, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol Kowloon Hong Kong Peoples R China s R China
Titolo Testata:
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
fascicolo: 1, volume: 295, anno: 2000,
pagine: 168 - 176
SICI:
0022-3565(200010)295:1<168:GCMTAA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; SIGNAL-REGULATED KINASE; BETA-GAMMA-SUBUNITS; ELEMENT-BINDING PROTEIN; HAMSTER OVARY CELLS; RECEPTOR INTERNALIZATION; G(BETA-GAMMA) SUBUNITS; INHIBITORY RECEPTORS; LOCUS-COERULEUS; MAP KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Wong, YH Hong Kong Univ Sci & Technol, Dept Biochem, Clear Water Bay, Kowloon, HongKong, Peoples R China Hong Kong Univ Sci & Technol Clear Water BayKowloon Hong Kong Peoples R China
Citazione:
P.H. Tso e Y.H. Wong, "Gz can mediate the acute actions of mu- and kappa-opioids but is not involved in opioid-induced adenylyl cyclase supersensitization", J PHARM EXP, 295(1), 2000, pp. 168-176

Abstract

The three subtypes of opioid receptors ( delta, mu, and kappa) are known to regulate multiple effectors through either pertussis toxin-sensitive or -insensitive G proteins. In opioid-induced inhibition of adenylyl cyclase, both G(i) and G(z) proteins can serve as the signal transducer. Our previousstudy showed that opioid-induced adenylyl cyclase supersensitization in human embryonic kidney (HEK) 293 cells expressing the delta-opioid receptor requires G(i) but not G(z) proteins. Herein, we studied the ability of mu-and and kappa-opioid receptors to regulate the activities of adenylyl cyclasethrough G(z). In HEK 293 cells coexpressing G(z) with the mu- or kappa-opioid receptors, opioid agonists induced inhibition of adenylyl cyclase in a pertussis toxin-insensitive manner. However, adenylyl cyclase supersensitization induced by chronic opioid treatments remained sensitive to pertussis toxin. We also showed that the responsiveness of cAMP-dependent response element-binding proteins to forskolin was not altered after prolonged opioid treatment but was higher in cells coexpressing G(z). Although the mu- and kappa-opioid receptors mediated acute activation of extracellular signal-regulated protein kinase 1/2 via both G(i) and G(z), these responses were abolished by chronic opioid treatment. These studies showed that G(z) could mediate acute actions of mu- and kappa-opioids but G(z) alone was insufficientto mediate adenylyl cyclase supersensitization induced by the chronic activation of opioid receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:38:24