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Titolo:
Effect of suckling on NADPH-diaphorase (nitric oxide synthase, NOS) reactivity and NOS gene expression in the paraventricular and supraoptic nuclei of lactating rats
Autore:
Otukonyong, EE; Okere, CO; Johnstone, LE; Murata, T; Kaba, H; Higuchi, T;
Indirizzi:
Kochi Med Sch, Dept Physiol, Kochi 7838505, Japan Kochi Med Sch Kochi Japan 7838505 ch, Dept Physiol, Kochi 7838505, Japan Fukui Med Univ, Dept Physiol, Fukui, Japan Fukui Med Univ Fukui JapanFukui Med Univ, Dept Physiol, Fukui, Japan
Titolo Testata:
JOURNAL OF NEUROENDOCRINOLOGY
fascicolo: 10, volume: 12, anno: 2000,
pagine: 1001 - 1008
SICI:
0953-8194(200010)12:10<1001:EOSON(>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURONS IN-VITRO; OXYTOCIN SECRETION; FOS EXPRESSION; MESSENGER-RNA; UP-REGULATION; CGMP LEVELS; HYPOTHALAMUS; VASOPRESSIN; BRAIN; LOCALIZATION;
Keywords:
gene expression; lactation; nitric oxide synthase; paraventricular nucleus; supraoptic nucleus; suckling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Otukonyong, EE Kochi Med Sch, Dept Physiol, Kochi 7838505, Japan Kochi MedSch Kochi Japan 7838505 l, Kochi 7838505, Japan
Citazione:
E.E. Otukonyong et al., "Effect of suckling on NADPH-diaphorase (nitric oxide synthase, NOS) reactivity and NOS gene expression in the paraventricular and supraoptic nuclei of lactating rats", J NEUROENDO, 12(10), 2000, pp. 1001-1008

Abstract

This study examined the effect of suckling on nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d, a histochemical marker for nitric oxide synthase, NOS) reactivity and neuronal NOS mRNA expression in the paraventricular (PVN) and supraoptic (SON) nuclei of lactating rats. Freely nursing (non-separated) dams and those separated from pups for 12 h and then reunited for 0, 15, 30, 60, 90, 120 and 180 min were used for the study. Dams separated from pups and sacrificed at time zero (without reunion) showed a significant decrease in NADPH-d staining and NADPH-d positive cells as well as in the NOS mRNA expression in the PVN and SON compared to that observed in non-separated dams. Reunion with pups and restoration of suckling significantly increased NADPH-d reactivity after 15, 30, 60 min, but not after 90, 120 and 180 min compared to non-reunited pups-deprived dams. A pattern ofNADPH-d reactivity and neuronal NOS mRNA expression indistinguishable fromthat observed during free lactation was reinstated shortly (15 min) after the restoration of suckling stimulus, suggesting that the NADPH-d reactivity in lactation depends on the presence of the suckling stimulus. These results show that suckling stimulus may play a modulatory role in the regulation of NOS reactivity in the magnocellular neurones of the hypothalamic PVN and SON during lactation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:43:45