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Titolo:
Constitutive phosphorylation of the vesicular inhibitory amino acid transporter in rat central nervous system
Autore:
Bedet, C; Isambert, MF; Henry, JP; Gasnier, B;
Indirizzi:
Inst Biol Physicochim, CNRS, UPR 1929, F-75005 Paris, France Inst Biol Physicochim Paris France F-75005 R 1929, F-75005 Paris, France
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 4, volume: 75, anno: 2000,
pagine: 1654 - 1663
SICI:
0022-3042(200010)75:4<1654:CPOTVI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; SERINE THREONINE PHOSPHATASES; HUMAN DOPAMINE TRANSPORTER; SYNAPTIC VESICLES; NEUROTRANSMITTER TRANSPORTERS; ACETYLCHOLINE TRANSPORTER; CAENORHABDITIS-ELEGANS; MONOAMINE TRANSPORTER; GABA TRANSPORTER; SEROTONIN TRANSPORTERS;
Keywords:
synaptic vesicle; neurotransmitter transporter; gamma-aminobutyric acid; glycine; phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Gasnier, B Inst Biol Physicochim, CNRS, UPR 1929, 13 Rue Pierre & Marie Curie, F-75005 Paris, France Inst Biol Physicochim 13 Rue Pierre & Marie Curie Paris France F-75005
Citazione:
C. Bedet et al., "Constitutive phosphorylation of the vesicular inhibitory amino acid transporter in rat central nervous system", J NEUROCHEM, 75(4), 2000, pp. 1654-1663

Abstract

gamma-Aminobutyric acid (GABA) and glycine are stored into synaptic vesicles by a recently identified vesicular inhibitory amino acid transporter [VIAAT, also called vesicular GABA transporter (VGAT)], Immunoblotting analysis revealed that rat brain VIAAT migrated as a doublet during sodium dodecylsulfate-polyacrylamide gel electrophoresis, with a predominant slower bandin all areas examined except olfactory bulb and retina. The slower band corresponded to a phosphorylated form of VIAAT as it was converted to the faster one by treating brain homogenates with alkaline phosphatase or with an endogenous phosphatase identified as type 2A protein-serine/threonine phosphatase using okadaic acid, In contrast, the recombinant protein expressed in COS-7 or PC12 cells co-migrated with the faster band of the brain doubletand was insensitive to alkaline phosphatase. To investigate the influence of VIAAT phosphorylation on vesicular neurotransmitter loading, purified synaptic vesicles were treated with alkaline phosphatase and assayed for amino acid uptake, However, neither GABA nor glycine uptake was affected by VIAAT phosphorylation, These results indicate that VIAAT is constitutively phosphorylated on cytosolic serine or threonine residues in most, but not all,regions of the rat brain. This phosphorylation does not regulate the vesicular loading of GABA or glycine, suggesting that it is involved at other stages of the synaptic vesicle life cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 17:03:14