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Titolo:
Induction of glycerol phosphate dehydrogenase gene expression during seizure and analgesia
Autore:
Link, WA; Kauselmann, G; Mellstrom, B; Kuhl, D; Naranjo, JR;
Indirizzi:
CSIC, CNB, Dept Biol Mol & Celular, Madrid 28049, Spain CSIC Madrid Spain 28049 NB, Dept Biol Mol & Celular, Madrid 28049, Spain Univ Hamburg, Inst Neurale Signalverarbeitung, Zentrum Mol Neurobiol, Hamburg, Germany Univ Hamburg Hamburg Germany g, Zentrum Mol Neurobiol, Hamburg, Germany
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 4, volume: 75, anno: 2000,
pagine: 1419 - 1428
SICI:
0022-3042(200010)75:4<1419:IOGPDG>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINAL-CORD NEURONS; IMMEDIATE-EARLY GENE; MESSENGER-RNA; RAT-BRAIN; C-FOS; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; GLUCOCORTICOID REGULATION; CELL-LINE; SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE; ADIPOSE CONVERSION;
Keywords:
glycerol phosphate dehydrogenase; seizure; noxious stimulation; glucocorticoids; differential display; cycloheximide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Naranjo, JR CSIC, CNB, Dept Biol Mol & Celular, Campus UAM Cantoblanco, Madrid 28049, Spain CSIC Campus UAM Cantoblanco Madrid Spain 28049 d 28049, Spain
Citazione:
W.A. Link et al., "Induction of glycerol phosphate dehydrogenase gene expression during seizure and analgesia", J NEUROCHEM, 75(4), 2000, pp. 1419-1428

Abstract

Using mRNA differential display, we found that the gene for NAD(+)-dependent glycerol phosphate dehydrogenase (GPDH; EC 1.1.1.8) is induced in rat brain following seizure activity. Northern blot and in situ hybridization analysis confirmed the differential display results; they also showed, in a separate model of neuronal activation, that after thermal noxious stimulationof the hindpaws, a similar increase in GPDH mRNA occurs in the areas of somatotopic projection in the lumbar spinal cord, Surprisingly, administration of analgesic doses of morphine or the nonsteroidal antiinflammatory drugsaspirin, metamizol (dipyrone), and indomethacin also increased GPDH mRNA levels in rat spinal cord, The opioid receptor antagonist naloxone completely blocked morphine induction of GPDH but had no effect on GPDH induction bynoxious heat stimulation or metamizol treatment, implicating different mechanisms of GPDH induction. Nevertheless, in all cases, induction of the GPDH gene requires adrenal steroids and new protein synthesis, as the induction was blocked in adrenalectomized rats and by cycloheximide treatment, respectively. Our results suggest that the induction of the GPDH gene upon peripheral noxious stimulation is related to the endogenous response to pain asit is mimicked by exogenously applied analgesic drugs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 10:08:18