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Titolo:
The mammalian Rab family of small GTPases: Definition of family and subfamily sequence motifs suggests a mechanism for functional specificity in the Ras superfamily
Autore:
Pereira-Leal, JB; Seabra, MC;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Sch Med, Div Biomed Sci, Mol Genet Sect, London SW7 2AZ, England Univ London Imperial Coll Sci Technol &Med London England SW7 2AZ gland
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 4, volume: 301, anno: 2000,
pagine: 1077 - 1087
SICI:
0022-2836(20000825)301:4<1077:TMRFOS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUANINE-NUCLEOTIDE EXCHANGE; GTP-BINDING PROTEINS; STRUCTURAL BASIS; EFFECTOR DOMAIN; MUTATIONAL ANALYSIS; NUCLEAR TRANSPORT; CRYSTAL-STRUCTURE; TERMINAL DOMAIN; ARF GTPASE; ACTIVATION;
Keywords:
Rab proteins; Ras proteins; GTP; sequence motif; vesicular transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Seabra, MC Univ London Imperial Coll Sci Technol & Med, Sch Med, Div Biomed Sci, Mol Genet Sect, Sir Fleming Bldg, London SW7 2AZ, England Univ London Imperial Coll Sci Technol & Med Sir Fleming Bldg London England SW7 2AZ
Citazione:
J.B. Pereira-Leal e M.C. Seabra, "The mammalian Rab family of small GTPases: Definition of family and subfamily sequence motifs suggests a mechanism for functional specificity in the Ras superfamily", J MOL BIOL, 301(4), 2000, pp. 1077-1087

Abstract

The Rab/Ypt/Sec4 family forms the largest branch of the Ras superfamily ofGTPases, acting as essential regulators of vesicular transport pathways. We used the large amount of information in the databases to analyse the mammalian Rab family. We defined Rab-conserved sequences that we designate Rab family (RabF) motifs using the conserved PM and G motifs as "landmarks". The Rab-specific regions were used to identify new Rab proteins in the databases and suggest rules for nomenclature. Surprisingly, we find that RabF regions cluster in and around switch I and switch II regions, i.e. the regionsthat change conformation upon GDP or GTP binding. This finding suggests that specificity of Rab-effector interaction cannot be conferred solely through the switch regions as is usually inferred. Instead, we propose a model whereby an effector binds to RabF (switch) regions to discriminate between nucleotide-bound states and simultaneously to other regions that confer specificity to the interaction, possibly Rab subfamily (RabSF) specific regionsthat we also define here. We discuss structural and functional data that support this model and its general applicability to the Ras superfamily of proteins. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:52:51