Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The interconversion of isoforms of seminal ribonuclease: Modelling key intermediates and trypsin effects
Autore:
Cubellis, MV; DAlessio, G;
Indirizzi:
Univ Naples Federico II, Dipartimento Chim Organ & Biol, I-80134 Naples, Italy Univ Naples Federico II Naples Italy I-80134 Biol, I-80134 Naples, Italy
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 4, volume: 301, anno: 2000,
pagine: 775 - 782
SICI:
0022-2836(20000825)301:4<775:TIOIOS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIMITED PROTEOLYTIC SITES; BOVINE BETA-TRYPSIN; CRYSTAL-STRUCTURE; PROTEIN STRUCTURES; INHIBITOR; COMPLEX; REFINEMENT; RESOLUTION; GEOMETRY; DOMAIN;
Keywords:
BS-RNase; domain swapping; limited proteolysis; molecular modelling; molecular dynamics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: D'Alessio, G Univ Naples Federico II, Dipartimento Chim Organ & Biol, Via Mezzocannone 16, I-80134 Naples, Italy Univ Naples Federico II Via Mezzocannone 16 Naples Italy I-80134
Citazione:
M.V. Cubellis e G. D'Alessio, "The interconversion of isoforms of seminal ribonuclease: Modelling key intermediates and trypsin effects", J MOL BIOL, 301(4), 2000, pp. 775-782

Abstract

The stepwise tryptic degradation of the interconverting quaternary isoforms of seminal ribonuclease has been analysed by structural modelling, based on the experimental results obtained by treating the dimeric protein with trypsin. The results of the analysis were compared with those obtained applying to the action of trypsin on seminal ribonuclease a recently proposed predictive algorithm for limited proteolysis. The attention was focussed on the MxM form of the protein, in which the two subunits swap their N-terminalends interconverting at equilibrium with the M=M form with no interchange between subunits. The analysis led to the identification of a key intermediate in the interconversion pathway, and to the resolution of the apparent contradiction between prediction and actual experimental data. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 03:11:27