Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Peak shapes in open tubular ion-exchange capillary electrochromatography of inorganic anions
Autore:
Breadmore, MC; Boyce, M; Macka, M; Avdalovic, N; Haddad, PR;
Indirizzi:
Univ Tasmania, Sch Chem, Hobart, Tas 7001, Australia Univ Tasmania HobartTas Australia 7001 Chem, Hobart, Tas 7001, Australia Edith Cowan Univ, Perth, WA 6050, Australia Edith Cowan Univ Perth WA Australia 6050 Univ, Perth, WA 6050, Australia Dionex Corp, Sunnyvale, CA 94086 USA Dionex Corp Sunnyvale CA USA 94086Dionex Corp, Sunnyvale, CA 94086 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 892, anno: 2000,
pagine: 303 - 313
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUSED-SILICA CAPILLARIES; LIQUID-CHROMATOGRAPHY; STATIONARY-PHASE; ELECTROPHORESIS; SEPARATIONS; SELECTIVITY; PROTEINS; SURFACE; COLUMNS; FLOW;
Keywords:
peak shapes; open tubular ion-exchange capillary electrochromatography; electrochromatography; inorganic anions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Haddad, PR Univ Tasmania, Sch Chem, GPO Box 252-75, Hobart, Tas 7001, Australia Univ Tasmania GPO Box 252-75 Hobart Tas Australia 7001 ustralia
Citazione:
M.C. Breadmore et al., "Peak shapes in open tubular ion-exchange capillary electrochromatography of inorganic anions", J CHROMAT A, 892(1-2), 2000, pp. 303-313

Abstract

An experimental study of parameters influencing peak shapes in ion-exchange open tubular (OT) capillary electrochromatography (CEC) was conducted using adsorbed quaternary aminated latex particles as the stationary phase. The combination of separation mechanisms from both capillary electrophoresis and ion-exchange chromatography results in peak broadening in OT-CEC arising from both these techniques. The sources of peak broadening that were considered included the relative electrophoretic mobilities of the eluent co-ion and analyte, and resistance to mass transfer in both the mobile and stationary phases. The parameters investigated were the mobility of the eluent co-ion, column diameter, separation temperature and secondary interactions between the analyte and the stationary phase. The electromigration dispersion was found to influence peak shapes to a minor extent, indicating that chromatographic retention was the dominant source of dispersion. Improving theresistance to mass transfer in the mobile phase by decreasing the capillary diameter improved peak shapes, with symmetrical peaks being obtained in a25 mu m I.D. column. However, an increase in temperature from 25 degrees Cto 55 degrees C failed to show any significant improvement. The addition of p-cyanophenol to the mobile phase to suppress secondary interactions withthe stationary phase did not result in the expected improvement in efficiency. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 06:01:36