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Titolo:
A method for measuring specific IgE in sera by direct ELISA without interference by IgG competition or IgG autoantibodies to IgE
Autore:
Kadooka, Y; Idota, T; Gunji, H; Shimatani, M; Kawakami, H; Dosako, S; Samori, T;
Indirizzi:
Snow Brand Milk Prod Co Ltd, Nutr Sci Lab, Kawagoe, Saitama 3501165, JapanSnow Brand Milk Prod Co Ltd Kawagoe Saitama Japan 3501165 3501165, Japan Samori Childrens Clin, Osaka, Japan Samori Childrens Clin Osaka JapanSamori Childrens Clin, Osaka, Japan
Titolo Testata:
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
fascicolo: 4, volume: 122, anno: 2000,
pagine: 264 - 269
SICI:
1018-2438(200008)122:4<264:AMFMSI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-IGE; IMMUNE-COMPLEXES; PROTEIN-G; ATOPIC-DERMATITIS; BINDING-PROPERTIES; ANTIBODIES; ASSAY; CHILDREN; CAP;
Keywords:
specific IgE; ELISA; RAST; protein G; autoantibodies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kadooka, Y Snow Brand Milk Prod Co Ltd, Nutr Sci Lab, 1-1-2 Minamidai, Kawagoe, Saitama 3501165, Japan Snow Brand Milk Prod Co Ltd 1-1-2 Minamidai Kawagoe Saitama Japan 3501165
Citazione:
Y. Kadooka et al., "A method for measuring specific IgE in sera by direct ELISA without interference by IgG competition or IgG autoantibodies to IgE", INT A AL IM, 122(4), 2000, pp. 264-269

Abstract

Background In measuring specific IgE levels in sera by direct ELISA, competition with coexisting IgG often impedes an exact IgE determination; additionally, IgG autoantibodies to IgE (IgG-IgE) in sera affect the assay. in this paper, we attempt to determine accurate specific IgE levels by selectiveremoval of IgG with a protein G-immobilized gel (PG) and by acid treatmentof the PG to compensate for the unintended removal of IgE, probably due tothe PG binding IgG-IgE, Methods: IgG in sera was removed using PG at pH 7.0. Then, the PG was treated with citrate buffer at pH 3.0 for 5 min to liberate IgE from IgG-IgE complexes, after IgG-binding sites on the PG were saturated with bovine IgG, since PG came to bind IgE at acidic pi-is. IgE levels were then measured by ELISA. Results: The PG treatment of sera removed the effect of inhibitory competition by coexisting IgG, especially at higherconcentrations of sera, to improve specific IgE detection by direct ELISA,However, PG treatment alone sometimes reduced IgE levels (39% of sera tested), even though PG does not bind IgE at pH 7.0, which indicated the presence of IgG-IgE complexes. The reduction in IgE returned almost to their original levels in the sera by acid treatment of the PG, By combining the PG treatment with acid treatment, specific IgE measurement in sera was improved significantly (p < 0.01, Wilcoxon signed rank test). Conclusion: Measurement of specific IgE in sera by direct ELISA was improved by using the PG and acid treatment technique. Copyright (C) 2000 S. Karger AG,Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:21:45