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Titolo:
Clinical significance of expression of membrane type 1 matrix metalloproteinase and matrix metalloproteinase-2 in human head and neck squamous cell carcinoma
Autore:
Imanshi, V; Fujii, M; Tokumaru, Y; Tomita, T; Kanke, M; Kanzaki, J; Kameyama, K; Otani, Y; Sato, H;
Indirizzi:
Keio Univ, Sch Med, Dept Otolaryngol, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 aryngol, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ, Sch Med, Dept Pathol, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 Sch Med, Dept Pathol, Tokyo 1608582, Japan Keio Univ, Sch Med, Dept Surg, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 , Sch Med, Dept Surg, Tokyo 1608582, Japan Kanazawa Univ, Sch Med, Dept Mol Virol & Oncol, Canc Res Inst, Kanazawa, Ishikawa 920, Japan Kanazawa Univ Kanazawa Ishikawa Japan 920 , Kanazawa, Ishikawa 920, Japan
Titolo Testata:
HUMAN PATHOLOGY
fascicolo: 8, volume: 31, anno: 2000,
pagine: 895 - 904
SICI:
0046-8177(200008)31:8<895:CSOEOM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPH-NODE METASTASES; PROGELATINASE-A; MESSENGER-RNA; HEPATOCELLULAR CARCINOMAS; EXTRACELLULAR-MATRIX; PROGNOSTIC FACTORS; CDNA LIBRARY; MT-MMP; ACTIVATION; GENE;
Keywords:
MT1-MMP; MMP-2; HNSCC; lymph node metastasis; multivariate logistic regression analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Fujii, M Keio Univ, Sch Med, Dept Otolaryngol, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan Keio Univ 35 Shinanomachi Tokyo Japan 1608582 kyo 1608582, Japan
Citazione:
V. Imanshi et al., "Clinical significance of expression of membrane type 1 matrix metalloproteinase and matrix metalloproteinase-2 in human head and neck squamous cell carcinoma", HUMAN PATH, 31(8), 2000, pp. 895-904

Abstract

Three different membrane-type matrix metalloproteinases (MT-MMPs) activatein vitro the latent form of matrix metalloproteinase-2 (MMP-2), which is one of the key proteinases in invasion and metastasis of various cancers. Weexamined the mRNA expression of MTI, 2, and 3-MMPs and MMP-2 in cell linesof head and neck squamous cell carcinoma (HNSCC) and quantitated the relative expression levels in human HNSCC tissues by Northern blotting. The tissue localization of MT1-MMP and MMP-2 was determined by immunohistochemistryand in situ hybridization. Their implications in clinicopathologic factorswere statistically evaluated. All cell lines examined consistently expressed MT1-MMP and MMP-2, but not MT2, 3-MMP. In the clinical specimens, there was a significant correlation in coexpression of messenger of RNA (P = .0005) and colocalization by immunohistochemistry (P < .0001) for MT1-MMP and MMP-2. Relative mRNA expression levels of MT1-MMP and MMP-2 in the carcinomatissues were significantly higher than those of the control tissues (P = .0045 and P = .0122, respectively). Both mRNA expression level and immunopositivity of MT1-MMP significantly correlated with lymph node metastasis (P =.0081 and P = .0193, respectively) which was confirmed by multivariate logistic regression analysis. Immunoreaction of MT1-MMP and its mRNA expression were observed in both carcinoma cells and stromal cells. The localizationof MMP-2 closely corresponded to that of MT1-MMP. These observations suggest that MT1-MMP possesses a role as a determinant of lymph node metastasis in HNSCC, and that concurrent expression of MT1-MMP and MMP-2 are involved in progression of HNSCC. Copyright (C) 2000 by W.B. Saunders Company.

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Documento generato il 14/07/20 alle ore 10:00:21