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Titolo:
Genomic organization, chromosomal localization and regulation of expression of the neuronal nuclear matrix protein NRP/B in human brain tumors
Autore:
Kim, TA; Ota, S; Jiang, SX; Pasztor, LM; White, RA; Avraham, S;
Indirizzi:
Beth Israel Deaconess Med Ctr, Harvard Inst Med, Div Expt Med, Boston, MA 02115 USA Beth Israel Deaconess Med Ctr Boston MA USA 02115 d, Boston, MA 02115 USA Univ Missouri, Childrens Mercy Hosp, Sch Med, Sect Med Genet & Mol Med, Kansas City, MO 64108 USA Univ Missouri Kansas City MO USA 64108 Mol Med, Kansas City, MO 64108 USA
Titolo Testata:
GENE
fascicolo: 1, volume: 255, anno: 2000,
pagine: 105 - 116
SICI:
0378-1119(20000905)255:1<105:GOCLAR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERACTION MOTIF; DOMAIN; CANCER; GENE; DIFFERENTIATION; MORPHOGENESIS; BINDING; ENCODES; ENC-1; SPERM;
Keywords:
BTB/POZ domain; chromosome 5q; human brain tumor; Kelch motif; nuclear matrix protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Avraham, S Beth Israel Deaconess Med Ctr, Harvard Inst Med, Div Expt Med, 4 Blackfan Circle, Boston, MA 02115 USA Beth Israel Deaconess Med Ctr 4 Blackfan Circle Boston MA USA 02115
Citazione:
T.A. Kim et al., "Genomic organization, chromosomal localization and regulation of expression of the neuronal nuclear matrix protein NRP/B in human brain tumors", GENE, 255(1), 2000, pp. 105-116

Abstract

The nuclear matrix and its role in cell physiology are largely unknown, and the discovery of any matrix constituent whose expression is tissue- and/or cell-specific offers a new avenue of exploration. Studies of the novel neuronal nuclear matrix protein, NRP/B, reveal that it is an early and highlyspecific marker of neuronal induction and development in vertebrates, since its expression is restricted mainly to the developing and mature nervous system. These studies also show that NRP/B is involved in neuronal differentiation. To further examine the structure-function of NRP/B, we have clonedand characterized the murine Nrp/b gene. The murine gene consists of four exons interrupted by three introns that span 7.6 kb of DNA. The complete open reading frame is localized in exon 3, suggesting that NRP/B is highly conserved during evolution. Chromosomal analysis shows that NRP/B is localized to chromosome 13 in mouse and chromosome 5q12-13 in human. Since our previous studies demonstrated that NRP/B is expressed in primaryhippocampal neurons but not in primary astrocytes, we have characterized NRP/B mRNA and protein expression in various brain cell lines and in human brain tumors. Abundant expression of NRP/B mRNA and protein was observed in human neuroblastoma cell lines (IMR32, SKN-MC, SKN-SH), in glioblastoma cell lines (A172, T98G, U87-MG, U118-MG, U138-MG, and U373-MG), in neuroglioma(H4) and astrocytoma cell lines (CCF-STTG1 and SW1088). Confocal analysis of NRP/B in U87-MG glioblastoma cells indicated nuclear localization of NRP/B. NRP/B expression was also observed in human primary brain tumors including glioblastoma multiformae and astrocytomas (total of five cases). These results suggest that NRP/B expression is upregulated in human brain tumors including glioblastomas and astrocytomas, while under normal conditions NRP/B expression is restricted to neurons. This study implicates a role for NRP/B in brain tumor development. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 07/07/20 alle ore 05:54:38