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Titolo:
Enhanced oxidative stress and accelerated cellular senescence in glucose-6-phosphate dehydrogenase (G6PD)deficient human fibroblasts
Autore:
Ho, HY; Cheng, ML; Lu, FJ; Chou, YH; Stern, A; Liang, CM; Chiu, DTY;
Indirizzi:
Acad Sinica, Inst Biol Chem, Nankang, Taiwan Acad Sinica Nankang TaiwanAcad Sinica, Inst Biol Chem, Nankang, Taiwan Natl Def Med Ctr, Grad Isnt Life Sci, Taipei, Taiwan Natl Def Med Ctr Taipei Taiwan Ctr, Grad Isnt Life Sci, Taipei, Taiwan Chang Gung Univ, Grad Inst Basic Med Sci, Tao Yuan, Taiwan Chang Gung Univ Tao Yuan Taiwan ad Inst Basic Med Sci, Tao Yuan, Taiwan Chang Gung Univ, Sch Med Technol, Tao Yuan, Taiwan Chang Gung Univ Tao Yuan Taiwan Univ, Sch Med Technol, Tao Yuan, Taiwan Natl Taiwan Univ, Coll Med, Dept Biochem & Internal Med, Taipei 10018, Taiwan Natl Taiwan Univ Taipei Taiwan 10018 Internal Med, Taipei 10018, Taiwan Chang Gung Childrens Hosp, Dept Neonatol, Tao Yuan, Taiwan Chang Gung Childrens Hosp Tao Yuan Taiwan pt Neonatol, Tao Yuan, Taiwan New York Med Ctr, Dept Pharmacol, New York, NY USA New York Med Ctr New York NY USA d Ctr, Dept Pharmacol, New York, NY USA
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 2, volume: 29, anno: 2000,
pagine: 156 - 169
SICI:
0891-5849(20000715)29:2<156:EOSAAC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIFE-SPAN; MOLECULAR CHARACTERIZATION; DROSOPHILA-MELANOGASTER; SUPEROXIDE-DISMUTASE; BASE SUBSTITUTIONS; HYDROGEN-PEROXIDE; INVITRO LIFESPAN; G6PD DEFICIENCY; HUMAN-CELLS; DNA-DAMAGE;
Keywords:
glucose-6-phosphate dehydrogenase (G6PD); cell growth; cell senescence; oxidative stress; free radicals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Chiu, DTY Acad Sinica, Inst Biol Chem, 138,Sec 2,Acad Sinica Rd, Nankang, Taiwan Acad Sinica 138,Sec 2,Acad Sinica Rd Nankang Taiwan ng, Taiwan
Citazione:
H.Y. Ho et al., "Enhanced oxidative stress and accelerated cellular senescence in glucose-6-phosphate dehydrogenase (G6PD)deficient human fibroblasts", FREE RAD B, 29(2), 2000, pp. 156-169

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) is involved in the generation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and the maintenance of the cellular redox balance. The biological effects of G6PD deficiency in nucleated cells were studied using G6PD-deficient human foreskin fibroblasts (HFF). in contrast to that of normal HFF, the doubling Lime of G6PD-deficient cells increased readily from population doubling level (PDL) 15 to 63. This was accompanied by a significant increase in the percentage of G(1) cells. The slow-down in growth preceded an early entry of these cells into a nondividing state reminiscent of cellular senescence. These cells exhibited a significant increase in level of senescence-associated beta-galactosidase (SA-beta-gal) staining. The importance of G6PD activity in cell growth was corroborated by the finding that ectopic expression of active G6PDin the deficient cells prevented their growth retardation and early onset of senescence. Mechanistically, the enhanced fluorescence in dichlorofluorescin (H2DCF)-stained G6PD-deficient cells suggests the possible involvementof reactive oxygen species in senescence. Taken together, our results showthat G6PD deficiency predisposes human fibroblasts to retarded growth and accelerated cellular senescence. Moreover, G6PD-deficient HFF provides a useful model system for delineating the effects of redox alterations on cellular processes. (C) 2000 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:42:03