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Titolo:
Trypanosoma cruzi: A putative vacuolar ATP synthase subunit and a CAAX prenyl protease-encoding gene, as examples of gene identification in genome projects
Autore:
Porcel, BM; Aslund, L; Pettersson, U; Andersson, B;
Indirizzi:
Rudbeck Lab, Dept Genet & Pathol, Med Genet Sect, SE-75185 Uppsala, SwedenRudbeck Lab Uppsala Sweden SE-75185 Genet Sect, SE-75185 Uppsala, Sweden
Titolo Testata:
EXPERIMENTAL PARASITOLOGY
fascicolo: 3, volume: 95, anno: 2000,
pagine: 176 - 186
SICI:
0014-4894(200007)95:3<176:TCAPVA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
H+-ATPASE; A-FACTOR; ENDOPLASMIC-RETICULUM; SCHISTOSOMA-MANSONI; PLASMA-MEMBRANE; 14-KDA SUBUNIT; PROTEINS; YEAST; STE24P; PROTEOLYSIS;
Keywords:
Trypanosoma cruzi; Homo sapiens; Saccharomyces cerevisiae; Arabidopsis thaliana; Plasmodium falciparum; Trypanosoma brucei; Schistosoma mansoni; Caenorhabditis elegans; Manduca sexta; Leishmania major; Drosophila melanogaster; Anopheles gambiae; Schizosaccharomyces pombe; Methanobacterium thermoautotrophicum; Pyrococcus horikoshii; Helicobacter pylori; Bacillus subtilis; genome project;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Andersson, B Rudbeck Lab, Dept Genet & Pathol, Med Genet Sect, SE-75185 Uppsala, Sweden Rudbeck Lab Uppsala Sweden SE-75185 E-75185 Uppsala, Sweden
Citazione:
B.M. Porcel et al., "Trypanosoma cruzi: A putative vacuolar ATP synthase subunit and a CAAX prenyl protease-encoding gene, as examples of gene identification in genome projects", EXP PARASIT, 95(3), 2000, pp. 176-186

Abstract

An international genome program has been initiated to increase the knowledge about the Trypanosoma cruzi genome and thereby find effective tools to treat Chagas' disease. We here report the molecular characterization of two novel genes found in the course of this project. Two of the open reading frames (ORF) identified in the sequencing of the third smallest chromosome ofthe CL Brener strain of T. cruzi were selected for further molecular characterization due to their similarity to genes with interesting functions in other organisms and their potential as targets to combat the parasite. The first ORF (402 bp) showed homology to a 14-kDa vacuolar ATP synthase subunit F from a variety of organisms, such as yeast, rat, bovine, human, and a number of prokaryotes. The second ORF (1188 bp) resembled a CAAX prenyl protease-encoding gent, identified in different organisms, including Homo sapiens, Saccharomyces cerevisiae, and Arabidopsis thaliana, as well as several prokaryotes. RT-PCR from T. cruzi total epimastigote RNA allowed us to isolate the complete transcripts: of these genes. Furthermore, screening of an available normalized cDNA library derived from the same stage of the parasite confirmed that both genes are expressed at least in the epimastigote stage of T. cruzi. Comparison of the putative T. cruzi proteins to their counterparts in other organisms revealed significant protein sequence conservation over large evolutionary distances. Computer analysis revealed the presence of several motifs in both proteins, possibly related to the regulation and localization of these proteins in the parasite. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:45:16