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Titolo:
Expression and activation of caspase-3/CPP32 in CD34(+) cord blood cells is linked to apoptosis after growth factor withdrawal
Autore:
Wang, LS; Liu, HJ; Xia, ZB; Broxmeyer, HE; Lu, L;
Indirizzi:
Indiana Univ, Sch Med, Walther Oncol Ctr, Dept Immunol Microbiol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 robiol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Walther Oncol Ctr, Dept Med Hematol Oncol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 Oncol, Indianapolis, IN 46202 USA Walther Canc Inst, Indianapolis, IN USA Walther Canc Inst Indianapolis INUSA er Canc Inst, Indianapolis, IN USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 8, volume: 28, anno: 2000,
pagine: 907 - 915
SICI:
0301-472X(200008)28:8<907:EAAOCI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; NECROSIS-FACTOR-ALPHA; CRMA-INHIBITABLE PROTEASE; FAS ANTIGEN CD95; NF-KAPPA-B; ICE/CED-3-LIKE PROTEASE; SUPPRESSING APOPTOSIS; FUNCTIONAL EXPRESSION; INTERFERON-GAMMA; CPP32 PROTEASE;
Keywords:
caspase-3/CPP32; CD34(+) cells; cord blood; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, L Indiana Univ, Sch Med, Walther Oncol Ctr, Dept Immunol Microbiol, 1044 W Walnut St,Room 302, Indianapolis, IN 46202 USA Indiana Univ 1044 W Walnut St,Room 302 Indianapolis IN USA 46202 USA
Citazione:
L.S. Wang et al., "Expression and activation of caspase-3/CPP32 in CD34(+) cord blood cells is linked to apoptosis after growth factor withdrawal", EXP HEMATOL, 28(8), 2000, pp. 907-915

Abstract

Objective. Caspase-3/CPP32, a member of the interleukin-l converting enzyme (ICE) family, is considered an executioner protease in mammalian cells during apoptosis, Although expression and activation of caspase-3/CPP32 protein have been studied in many tissues and leukemia cell lines, this has not been explored in primitive hematopoietic CD34(+) cells. In this study, we evaluated expression and activation of caspase-3/CPP32 protein in CD34(+) cells from cord blood (CB) during apoptosis induced by growth factor deprivation. Methods. Reverse transcriptase-polymerase chain reaction (RT-PCR), Westernblot, and now cytometry analysis were used in this study to determine the expression of caspase-3/CPP32 in CD34(+) CB cells during apoptosis,Results. Our results demonstrated that caspase-3/CPP32 mRNA was constitutively expressed at a very low level in freshly isolated CD34(+) cells. Expression of caspase-3/CPP32 mRNA and protein was upregulated when these cells were first expanded in suspension culture with growth factors for 3 days. However, only the 32 kDa inactive caspase-3/CPP32 proenzyme was detected in the freshly isolated CD34(+) cells and after 3 days expansion with cytokines, Within 12 hours after growth factor withdrawal from expanded cells caspase-3/CPP32 was activated and a cleavage 20 kDa protein was detected; a poly(ADP-ribose) polymerase (PARP) was cleaved by activated caspase-3/CPP32, Activation of caspase-3/CPP32 and apoptosis upon growth factor withdrawal were inhibited/reduced by the caspase inhibitors, z-VAD-fmk and DEVD-CHO. Conclusion. These results demonstrate that caspase-3/CPP32 is involved in apoptosis of primitive Cn CD34(+) cells but may not be the only mechanism involved. (C) 2000 International Society for Experimental Hematology, Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 13:46:31