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Titolo:
C/EBP beta enhances IL-4 but impairs IL-2 and IFN-gamma induction in T cells
Autore:
Berberich-Siebelt, F; Klein-Hessling, S; Hepping, N; Santner-Nanan, B; Lindemann, D; Schimpl, A; Berberich, I; Serfling, E;
Indirizzi:
Univ Wurzburg, Inst Pathol, Dept Mol Pathol, D-97080 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97080 Pathol, D-97080 Wurzburg, Germany Univ Wurzburg, Inst Virol & Immunobiol, D-97080 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97080 nobiol, D-97080 Wurzburg, Germany
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 9, volume: 30, anno: 2000,
pagine: 2576 - 2585
SICI:
0014-2980(200009)30:9<2576:CBEIBI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-AT; INTERLEUKIN-4 PROMOTER; TRANSCRIPTION FACTORS; GENE-EXPRESSION; DEFICIENT MICE; DNA-BINDING; IN-VITRO; FAMILY; ACTIVATION; ELEMENTS;
Keywords:
C/EBP; lymphokine; NF-AT; transcription factor; GFP retrovirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Serfling, E Univ Wurzburg, Inst Pathol, Dept Mol Pathol, Josef Schneider Str 2, D-97080 Wurzburg, Germany Univ Wurzburg Josef Schneider Str 2 Wurzburg Germany D-97080
Citazione:
F. Berberich-Siebelt et al., "C/EBP beta enhances IL-4 but impairs IL-2 and IFN-gamma induction in T cells", EUR J IMMUN, 30(9), 2000, pp. 2576-2585

Abstract

C/EBP transcription factors have been described to control the activity ofthe human IL-4 promoter. The C/EBP binding sites within the IL-4 promoter overlap with composite NF-AT and AP-1 binding motifs. We show here that similar binding sites are part of the murine IL-4 promoter. Retroviral overexpression of C/EBP beta in murine EL-4 thymoma cells led to a strong induction of endogenous IL-4 and a reduction in IL-2 and IFN-gamma expression. Similarily, in primary murine T cells C/EBP beta induction resulted in an increase in IL-4 levels, whereas in human Jurkat T cells a decrease in IL-2 RNA was detected. Like AP-1, C/EBP factors belong to the large class of basic leucine zipper proteins. However, unlike AP-1, C/EBP beta does not act in synergy with NF-AT in the induction of the murine IL-4 promoter. instead, both factors compete in their binding to the P4/Pu-b(D) site, one of the most important sequence elements of the IL-4 promoter. Whereas NF-AT factors require high levels of free Ca2+ and calcineurin activity for induction, C/EBPinduction in T cells is Ca2+/calcineurin independent. These observations suggest that various induction conditions lead to the activation of transcription factors, inducing IL-4 promoter activity at specific developmental stages of T cells.

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Documento generato il 04/04/20 alle ore 09:07:46