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Titolo:
A novel Golgi membrane protein is part of a GTPase-binding protein complexinvolved in vesicle targeting
Autore:
Matern, H; Yang, XP; Andrulis, E; Sternglanz, R; Trepte, HH; Gallwitz, D;
Indirizzi:
Max Planck Inst Biophys Chem, Dept Mol Genet, D-37070 Gottingen, Germany Max Planck Inst Biophys Chem Gottingen Germany D-37070 ottingen, Germany SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA SUNYStony Brook Stony Brook NY USA 11794 Biol, Stony Brook, NY 11794 USA
Titolo Testata:
EMBO JOURNAL
fascicolo: 17, volume: 19, anno: 2000,
pagine: 4485 - 4492
SICI:
0261-4189(20000901)19:17<4485:ANGMPI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST SECRETORY PATHWAY; CIS-GOLGI; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; YPT1 PROTEIN; TRANSPORT; SNARE; IDENTIFICATION; LOCALIZATION; EXOCYTOSIS;
Keywords:
Golgi; vesicular transport; Yif1p-Yip1p complex; Ypt GTPases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Gallwitz, D Max Planck Inst Biophys Chem, Dept Mol Genet, D-37070 Gottingen, Germany Max Planck Inst Biophys Chem Gottingen Germany D-37070 rmany
Citazione:
H. Matern et al., "A novel Golgi membrane protein is part of a GTPase-binding protein complexinvolved in vesicle targeting", EMBO J, 19(17), 2000, pp. 4485-4492

Abstract

Through two-hybrid interactions, protein affinity and localization studies, we previously identified Yip1p, an integral yeast Golgi membrane protein able to bind the Ras-like GTPases Ypt1p and Ypt31p in their GDP-bound conformation. In a further two-hybrid screen, we identified Yif1p as an interacting factor of Yip1p, We show that Yif1p is an evolutionarily conserved, essential 35.5 kDa transmembrane protein that forms a tight complex with Yip1pon Golgi membranes. The hydrophilic N-terminal half of Yif1p faces the cytosol, and according to two-hybrid analyses can interact with the transport GTPases Ypt1p, Ypt31p and Sec4p, but in contrast to Yip1p, this interactionis dispensable for Yin protein function. Loss of Yif1p function in conditional-lethal mutants results in a block of endoplasmic reticulum (ER)-to-Golgi protein transport and in an accumulation of ER membranes and 40-50 nm vesicles. Genetic analyses suggest that Yif1p acts downstream of Yip1p, It isinferred that Ypt GTPase binding to the Yip1p-Yif1p complex is essential. for and precedes vesicle docking and fusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:16:28