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Titolo:
New aspects of buffering with multivalent weak acids in capillary zone electrophoresis: Pros and cons of the phosphate buffer
Autore:
Gebauer, P; Bocek, P;
Indirizzi:
Acad Sci Czech Republ, Inst Analyt Chem, CZ-61142 Brno, Czech Republic Acad Sci Czech Republ Brno Czech Republic CZ-61142 Brno, Czech Republic
Titolo Testata:
ELECTROPHORESIS
fascicolo: 14, volume: 21, anno: 2000,
pagine: 2809 - 2813
SICI:
0173-0835(200008)21:14<2809:NAOBWM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREDICTING PEAK SYMMETRY; BACKGROUND ELECTROLYTES;
Keywords:
phosphate buffer; electromigration dispersion; multivalent buffers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Bocek, P Acad Sci Czech Republ, Inst Analyt Chem, Veveri 97, CZ-61142 Brno, Czech Republic Acad Sci Czech Republ Veveri 97 Brno Czech Republic CZ-61142 lic
Citazione:
P. Gebauer e P. Bocek, "New aspects of buffering with multivalent weak acids in capillary zone electrophoresis: Pros and cons of the phosphate buffer", ELECTROPHOR, 21(14), 2000, pp. 2809-2813

Abstract

Phosphate buffer is frequently used as background electrolyte in capillaryelectrophoresis. It can cover a broad range of pH due to the three dissociation constants (pK(1) = 2.0, pK(2) -7.2, and pK(3) = 11.0) of phosphoric acid and because it is UV-light transparent. This contribution brings a theoretical study of the analytical separation performance (sample window, regions of peak symmetry, regions of fronting and tailing peaks) of phosphate buffer, serving as a model of buffering with multivalent weak acids. The study is based on the use of peak shape diagrams and covers the pH range 2.55-11.43. New important general knowledge has been revealed that single multivalent weak acids mimic the performance of background electrolytes with multiple co-anionic species for anionic analyses. It is shown that simple phosphate buffers prepared by mixing phosphoric acid and potassium hydroxide mayexhibit up to two regions of symmetry, of fronting as well as of tailing zones, on the mobility scale inside the sample window. Moreover they may exhibit a "schizophrenic" region of enormous electromigration dispersion.

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Documento generato il 06/07/20 alle ore 08:50:07