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Titolo:
Window optimization in isotachophoresis superimposed on capillary zone electrophoresis
Autore:
Beckers, JL;
Indirizzi:
Eindhoven Univ Technol, Dept Chem SPO, NL-5600 MB Eindhoven, Netherlands Eindhoven Univ Technol Eindhoven Netherlands NL-5600 MB ven, Netherlands
Titolo Testata:
ELECTROPHORESIS
fascicolo: 14, volume: 21, anno: 2000,
pagine: 2788 - 2796
SICI:
0173-0835(200008)21:14<2788:WOIISO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
STACKING;
Keywords:
isotachophoresis; capillary zone electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Beckers, JL Eindhoven Univ Technol, Dept Chem SPO, STO 3-25,POB 513, NL-5600 MB Eindhoven, Netherlands Eindhoven Univ Technol STO 3-25,POB 513 Eindhoven Netherlands NL-5600 MB
Citazione:
J.L. Beckers, "Window optimization in isotachophoresis superimposed on capillary zone electrophoresis", ELECTROPHOR, 21(14), 2000, pp. 2788-2796

Abstract

A sample stacking procedure to which a specific combination of electrolytesolutions is applied is isotachophoresis (ITP) superimposed on capillary zone electrophoresis (CZE), a so-called ITP/CZE system. In ITP/CZE some components migrate in an ITP fashion on top of a background electrolyte, and the other analytes migrate in a zone electrophoretic manner. For such a system, the leading electrolyte consists of a mixture of an ionic species, L1, of high mobility (the leading ion of the ITP system), an ionic species, L2, of low mobility (the colons of the CZE system), and a buffering counterionic species, whereas the terminating solution only contains the ionic speciesL2 and the buffering counterions. The nones of the components migrating inthe ITP/CZE mode are sharp owing to the self-correcting properties of the zones and the concentrations of the L1 ions of the system. Mobility windowscan be calculated, indicating which ions can migrate in the ITP/CZE mode. In this article mobility windows are calculated by applying both strong andweak acids as L1 and L2 ions and it appears that mobility windows can be optimized by chosing different ratios of L1 and L2 as well as different pH values. It is possible to construct very narrow mobility windows, and thereby choose which component of a sample solution can be concentrated, and to what concentration, in a very selective way. The big advantage of ITP/CZE compared with applications such as transient ITP and transient stacking is that the stacked sample ionic species migrate in the ITP mode during the whole experiment; furthermore, they do not destack. Experimentally obtained electropherograms validate the calculated mobility windows for the ITP/CZE mode.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:20:20