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Titolo:
Indirect spectrophotometric detection of inorganic anions in ion-exchange capillary electrochromatography
Autore:
Boyce, MC; Breadmore, M; Macka, M; Doble, P; Haddad, PR;
Indirizzi:
Univ Tasmania, Sch Chem, Hobart, Tas 7001, Australia Univ Tasmania HobartTas Australia 7001 Chem, Hobart, Tas 7001, Australia Edith Cowan Univ, Perth, WA, Australia Edith Cowan Univ Perth WA Australia ith Cowan Univ, Perth, WA, Australia
Titolo Testata:
ELECTROPHORESIS
fascicolo: 15, volume: 21, anno: 2000,
pagine: 3073 - 3080
SICI:
0173-0835(200009)21:15<3073:ISDOIA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIRECT PHOTOMETRIC DETECTION; ELECTROPHORESIS; CHROMATOGRAPHY; SEPARATION; ELECTROLYTES; CHROMATE;
Keywords:
capillary electrochromatography; indirect spectrophotometric detection; ion exchange-capillary electrochromatography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Haddad, PR Univ Tasmania, Sch Chem, GPO Box 252-75, Hobart, Tas 7001, Australia Univ Tasmania GPO Box 252-75 Hobart Tas Australia 7001 ustralia
Citazione:
M.C. Boyce et al., "Indirect spectrophotometric detection of inorganic anions in ion-exchange capillary electrochromatography", ELECTROPHOR, 21(15), 2000, pp. 3073-3080

Abstract

The application of indirect spectrophotometric detection was investigated for a capillary electrochromatographic system in which an anion-exchange stationary phase tin the form of aminated latex particles) was coated onto the wall of a fused-silica capillary. The study has focused on the choice of the type and concentration of the absorbing colon (probe) added to the background electrolyte and the role of this species in manipulating the ion-exchange contributions to the separation with a view to controlling the selectivity of the separation. Common inorganic anions were used as analytes and nitrate, p-toluenesulfonate, nicotinate, and chromate were investigated as probes. It was found that most of these probes produced only a limited range of separation selectivities when their concentration was varied over the practically accessible range. p-Toluenesulfonate provided the greatest variation in selectivity, but peak distortion due to electromigration dispersion was evident for the faster ions. When variation of the separation selectivity - from predominantly electrophoretic in nature to predominantly ion-exchange in nature - was desired, this was best achieved by varying the type of probe rather than its concentration. For example, the nitrate probe provided predominantly electrophoretic separations with good peak shapes and high efficiencies. A comprehensive list of probes, ranked in order of ion-exchange selectivity coefficients determined by ion chromatography, was compiled and this proved to be a useful tool to assist in the selection of a probe for a desired separation selectivity. The limits of detection for the analytes and probes studied ranged from 20-55 mu mol for the chromate system to 230-600 mu mol for the nicotinate system, with nitrate and p-toluenesulfonate giving intermediate values.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 15:52:15