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Titolo:
Purification of vitellin from grass shrimp Palaemonetes pugio, generation of monoclonal antibodies, and validation for the detection of lipovitellin in Crustacea
Autore:
Oberdorster, E; Rice, CD; Irwin, LK;
Indirizzi:
Clemson Univ, Dept Environm Toxicol, Pendelton, SC 29670 USA Clemson UnivPendelton SC USA 29670 ronm Toxicol, Pendelton, SC 29670 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY
fascicolo: 2, volume: 127, anno: 2000,
pagine: 199 - 207
SICI:
1367-8280(200009)127:2<199:POVFGS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALLINECTES-SAPIDUS; PENAEUS-VANNAMEI; DECAPODA; OVARIES; VITELLOGENESIS; CRAB; LIPOPROTEINS; MATURATION; HEMOLYMPH; PENAEIDAE;
Keywords:
blue crab; crayfish; Daphnia magna; grass shrimp; lipovitellin; mud crab; monoclonal antibody;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Oberdorster, E 509 Westinghouse Rd,POB 709, Pendelton, SC 29670 USA 509 Westinghouse Rd,POB 709 Pendelton SC USA 29670 670 USA
Citazione:
E. Oberdorster et al., "Purification of vitellin from grass shrimp Palaemonetes pugio, generation of monoclonal antibodies, and validation for the detection of lipovitellin in Crustacea", COMP BIOC C, 127(2), 2000, pp. 199-207

Abstract

Much effort has been put into developing vitellogenin antibodies against awide variety of aquatic vertebrate species to study potential estrogen or anti-estrogen endocrine disrupters. Little work has been done on endocrine disruption in aquatic invertebrates. Although some antibodies have been produced against blue crab and penaeid shrimp lipovitellin, they have only poor cross-reactivity with the important estuarine grass shrimp, Palaemonetes pugio. Vitellin was purified from eggs, monoclonal antibodies were producedusing standard techniques, and hybridoma supernatants were screened by ELISA. Western blots were done using extracts from male and female grass shrimp to verify specificity of the monoclonal antibodies. Two low molecular mass bands in the range of 68-85 kD and two high molecular mass bands in the range of 190-221 kD were found. In addition to grass shrimp, several other crustacean species were screened and cross-reactivity found, including blue crab (Callinectes sapidus), mud crab (Rhithropanopeus harrisii), red swamp crayfish (Procambarus clarkii) and Daphnia magna. To further investigate the use of the antibody, we performed a chronic 6-week pyrene exposure study. We found that vitellin was upregulated in females after 6 weeks and that this may be a protective measure against lipophilic xenobiotics. (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 11:25:43