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Titolo:
Concurrence of replicative senescence and elevated expression of p16(INK4A) with subculture-induced but not calcium-induced differentiation in normalhuman oral keratinocytes
Autore:
Lee, G; Park, BS; Han, SE; Oh, JE; You, YO; Baek, JH; Kim, GS; Min, BM;
Indirizzi:
Seoul Natl Univ, Coll Dent, Inst Dent Res, Dept Oral Biochem, Seoul 110749, South Korea Seoul Natl Univ Seoul South Korea 110749 chem, Seoul 110749, South Korea Seoul Natl Univ, Coll Dent, Inst Dent Res, Dept Pharmacol & Dent Therapeut, Seoul 110749, South Korea Seoul Natl Univ Seoul South Korea 110749 peut, Seoul 110749, South Korea
Titolo Testata:
ARCHIVES OF ORAL BIOLOGY
fascicolo: 10, volume: 45, anno: 2000,
pagine: 809 - 818
SICI:
0003-9969(200010)45:10<809:CORSAE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE EPIDERMAL-CELLS; NEOPLASTIC KERATINOCYTES; INTRACELLULAR CALCIUM; EXTRACELLULAR CALCIUM; TELOMERASE ACTIVITY; HUMAN FIBROBLASTS; P53; APOPTOSIS; CULTURE; IMMORTALIZATION;
Keywords:
calcium-induced differentiation; subculture-induced differentiation; normal human oral keratinocytes; replicative senesence; p16(INK4A);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Min, BM Seoul Natl Univ, Coll Dent, Inst Dent Res, Dept Oral Biochem, Seoul 110749, South Korea Seoul Natl Univ Seoul South Korea 110749 oul 110749, South Korea
Citazione:
G. Lee et al., "Concurrence of replicative senescence and elevated expression of p16(INK4A) with subculture-induced but not calcium-induced differentiation in normalhuman oral keratinocytes", ARCH ORAL B, 45(10), 2000, pp. 809-818

Abstract

Primary normal human oral keratinocytes (NHOKs) undergo differentiation inthe presence of calcium concentrations higher than 0.15 mM in vitro, whichis useful in investigating the mechanisms involved in the differentiation of epithelial cells. Serial subculture of NHOKs to the postmitotic stage also induces terminal differentiation. However, the detailed mechanisms of both differentiation processes remain substantially unknown. To investigate the molecular differences in these processes, NHOKs were induced to differentiate by exposure to 1.2 mM of calcium and by serial subculture to the postmitotic stage. To study whether the cells were induced to differentiate andto undergo replicative senescence, the amount of cellular involucrin and the expression of senescence-associated beta-galactosidase (SA-beta-gal) were measured respectively. The expression of replicative senescence-associated genes and the activity of telomerase from the differentiated cells were also determined. Both calcium treatment and serial subculture to the postmitotic stage notably elevated the cellular involucrin. The percentage of SA-beta-gal-positive cells was significantly elevated by the continued subculture, but such changes were not observed in keratinocytes exposed to calcium. The concentration of cellular p16(INK4A) protein was progressively increased by the continued subculture but was not changed by calcium treatment. Onthe other hand, the concentrations of cellular p53 were similar in both differentiation processes. However, telomerase activity was lost in NHOKs that had undergone differentiation by both calcium treatment and serial subculture. The results indicate that calcium-induced differentiation of NHOKs has similar characteristics to their serial subculture-induced differentiation, but that the differentiation processes are not identical, because calcium-induced differentiation does not concur with either replicative senescence or the gradually increased concentration of p16(INK4A). (C) 2000 Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:11:21