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Titolo:
Starvation increases the amount of pyruvate dehydrogenase kinase in several mammalian tissues
Autore:
Wu, PF; Blair, PV; Sato, J; Jaskiewicz, J; Popov, KM; Harris, RA;
Indirizzi:
Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USAIndiana Univ Indianapolis IN USA 46202 l Biol, Indianapolis, IN 46202 USA
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 1, volume: 381, anno: 2000,
pagine: 1 - 7
SICI:
0003-9861(20000901)381:1<1:SITAOP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM REGULATION; RAT MAMMARY-GLAND; STARVED-REFED TRANSITION; ACETYL-COA CARBOXYLASE; FATTY-ACID SYNTHESIS; COMPLEX; BRAIN; MITOCHONDRIA; INSULIN; PROTEIN;
Keywords:
pyruvate dehydrogenase complex; pyruvate dehydrogenase kinase; rat; starvation; liver; kidney; mammary gland; brown adipose tissue; adipose tissue; brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Harris, RA Indiana Univ, Sch Med, Dept Biochem & Mol Biol, 635 Barnhill Dr, Indianapolis, IN 46202 USA Indiana Univ 635 Barnhill Dr Indianapolis IN USA 46202 6202 USA
Citazione:
P.F. Wu et al., "Starvation increases the amount of pyruvate dehydrogenase kinase in several mammalian tissues", ARCH BIOCH, 381(1), 2000, pp. 1-7

Abstract

Covalent modification of the pyruvate dehydrogenase complex provides an important regulatory mechanism for controlling the disposal of glucose and other compounds metabolized to pyruvate. Regulation of the complex by this mechanism is achieved in part by tissue-specific expression of the genes encoding isoenzymes of pyruvate dehydrogenase kinase (PDK), Starvation is knownfrom our previous work to increase PDK activity of heart and skeletal muscle by increasing the amount of PDK isoenzyme 4 (PDK4) present in these tissues. This study demonstrates that increased expression of both PDK4 and PDK2 occurs in rat liver, kidney, and lactating mammary gland in response to starvation. PDK4 and PDK2 message levels were also increased by starvation in the two tissues examined (liver and kidney), suggesting enhancement of gene transcription. Changes in PDK2 message and protein were of similar magnitude, but changes in PDK4 message were greater than those in PDK4 protein, suggesting regulation at the level of translation. In contrast to these tissues, starvation had little or no effect on PDK2 and PDK4 protein in brain,white adipose tissue, and brown adipose tissue. Nevertheless, PDK4 messagelevels were significantly increased in brain and white adipose tissue by starvation. The findings of this study indicate that increased expression ofPDK isoenzymes is an. important mechanism for bringing about inactivation of the pyruvate dehydrogenase complex during starvation in many but not alltissues of the body. The absence of this mechanism preserves the capacity of neuronal tissue to utilize glucose for energy during starvation. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 05:24:48