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Titolo:
Activation of pig oocytes using calcium ionophore: effect of protein synthesis inhibitor cycloheximide
Autore:
Jilek, F; Huttelova, R; Petr, J; Holubova, M; Rozinek, J;
Indirizzi:
Czech Univ Agr, Dept Vet Sci, Prague 16521 6, Czech Republic Czech Univ Agr Prague Czech Republic 16521 6 gue 16521 6, Czech Republic Pronatal Sanatorium, IVF Unit, Prague 5, Czech Republic Pronatal Sanatorium Prague Czech Republic 5 it, Prague 5, Czech Republic Res Inst Anim Prod, Dept Reprod Biol, Prague 10, Czech Republic Res Inst Anim Prod Prague Czech Republic 10 l, Prague 10, Czech Republic
Titolo Testata:
ANIMAL REPRODUCTION SCIENCE
fascicolo: 1-2, volume: 63, anno: 2000,
pagine: 101 - 111
SICI:
0378-4320(20001002)63:1-2<101:AOPOUC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HISTONE H1 KINASE; MATURED IN-VITRO; PORCINE OOCYTES; BOVINE OOCYTES; PARTHENOGENETIC DEVELOPMENT; RABBIT OOCYTES; MAMMALIAN EGGS; FERTILIZATION; MATURATION; ETHANOL;
Keywords:
pig ovarium; oocyte activation; calcium ionophore; cycloheximide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Jilek, F Czech Univ Agr, Dept Vet Sci, Kamycka 129, Prague 16521 6, Czech Republic Czech Univ Agr Kamycka 129 Prague Czech Republic 16521 6 epublic
Citazione:
F. Jilek et al., "Activation of pig oocytes using calcium ionophore: effect of protein synthesis inhibitor cycloheximide", ANIM REPROD, 63(1-2), 2000, pp. 101-111

Abstract

In vitro matured pig oocytes were activated using a combined treatment of calcium ionophore A 23187 with cycloheximide. The oocytes were exposed to ionophore (10, 25 or 50 mu M) for 0.5, 1, 3, 5 or 7 min and then cultured with cycloheximide (0 or 10 mu g/ml) for 6 h. Cycloheximide treatment significantly increased the activation rate of oocytes and the percentage of oocytes that were able to develop after activation. The highest activation rate was observed after treatment with 50 mu M ionophore. The highest percentageof developing eggs was observed after combined treatment of ionophore (25 mu M) with cycloheximide. The percentage of oocytes developing up to the morula and blastocyst stage was not significantly increased after cycloheximide treatment. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 03:38:42