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Titolo:
In vitro culture of bovine preantral follicles
Autore:
Saha, S; Shimizu, M; Geshi, M; Izaike, Y;
Indirizzi:
Minist Agr Forestry & Fisheries, Natl Inst Anim Ind, Dept Anim Reprod, Ibaraki, Osaka 3050901, Japan Minist Agr Forestry & Fisheries Ibaraki Osaka Japan 3050901 050901, Japan
Titolo Testata:
ANIMAL REPRODUCTION SCIENCE
fascicolo: 1-2, volume: 63, anno: 2000,
pagine: 27 - 39
SICI:
0378-4320(20001002)63:1-2<27:IVCOBP>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; MOUSE OVARIAN FOLLICLES; EARLY ANTRAL FOLLICLES; LONG-TERM CULTURE; IN-VITRO; STIMULATING-HORMONE; GRANULOSA-CELLS; EXTRACELLULAR-MATRIX; DOMESTIC CATS; SECONDARY FOLLICLES;
Keywords:
cattle ovary; follicles; in vitro culture; in vitro growth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Saha, S Univ Tsukuba, Agr & Forestry Res Ctr, Tsukuba, Ibaraki 3058577, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058577 Ibaraki 3058577, Japan
Citazione:
S. Saha et al., "In vitro culture of bovine preantral follicles", ANIM REPROD, 63(1-2), 2000, pp. 27-39

Abstract

Bovine preantral follicles (40-100 mu m diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of thefour treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; + 1%) or FSH plus hypoxanthine (4 mM) in tissue culture medium(TCM 199) supplemented with 10% fetal calf serum (FCS), 0.1 mg/ml sodiumpyruvate, 100 IU/ml of penicillin acid 100 mu g/ml streptomycin. The control culture medium was TCM 199 with supplements without any treatments. Follicles of each size were cultured separately in groups of one to three in 24-well multidishes each containing 500 mu l of the appropriate culture medium. Culture commenced at follicle recovery (day 1) and continued for 10 days(harvested on day 11). In each case, half the medium was removed and replaced by fresh medium every third day. Follicle diameters were recorded on days 1, 5 and 11 of the experiment. At the end of the 10-day culture period, half of the follicles were stained with trypan blue to assess their potential viability and half were stained with bisbenzimide plus propidium iodine to estimate various morphological features of the follicles. Follicles of all initial sizes, on all culture treatments, increased in diameter during in vitro cultures with the greatest increases, both in absolute and proportional size, occurring between days 1 and 5 of culture. All ofthe culture medium supplements caused greater increases in follicle diameters than control medium at both days 5 and 11 of culture for all initial sizes of follicles (p < 0.01). The most effective culture supplements for follicles of 40-, 60- and 80-mu m initial diameter were FSH alone and FSH + EGF. The size of these follicles at both days 5 and 11 of culture on both thetreatments was significantly larger (p < 0.01) than follicles cultured in the presence of the other two supplementary treatments. The growth of follicles of 100-mu m initial diameter did not differ between culture medium supplements. None of the culture media caused follicle size to increase to theinitial diameters of the next larger size category during the 10 days of culture although follicles of 100-mu m diameter achieved a diameter of 120 mu m, after 4 days of culture. The overall follicular viability and morphology were better with treatments than the controls in all cases; however, there was no significant difference (p > 0.05) among them. From this experiment, FSH and FSH plus EGF may be recommended for in vitroculture of smaller (40, 60 and 80 mu m) follicles. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:18:39