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Titolo:
Potencies of naturally-occurring AKH/RPCH peptides in Locusta migratoria in the acetate uptake assay in vitro and comparison with their potencies in the lipid mobilisation assay in vivo
Autore:
Gade, G; Lee, MJ; Goldsworthy, GJ; Kellner, R;
Indirizzi:
Univ Cape Town, Dept Zool, ZA-7701 Rondebosch, South Africa Univ Cape Town Rondebosch South Africa ZA-7701 Rondebosch, South Africa Univ London Birkbeck Coll, Dept Biol, London WC1E 7HX, England Univ LondonBirkbeck Coll London England WC1E 7HX ndon WC1E 7HX, England Merck KGaA, Biomed F0 GBT, Darmstadt, Germany Merck KGaA Darmstadt Germany ck KGaA, Biomed F0 GBT, Darmstadt, Germany
Titolo Testata:
ACTA BIOLOGICA HUNGARICA
fascicolo: 2-4, volume: 51, anno: 2000,
pagine: 369 - 377
SICI:
0236-5383(2000)51:2-4<369:PONAPI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONCENTRATING HORMONE FAMILY; ADIPOKINETIC PEPTIDES; BIOLOGICAL-ACTIVITY; IN-VITRO; FAT-BODY; AKH-I; SPECTROSCOPY; RESIDUES; ANALOGS;
Keywords:
Locusta migratoria; acetate uptake assay; lipid mobilisation; AKH/RPCH peptides; receptor interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Gade, G Univ Cape Town, Dept Zool, ZA-7701 Rondebosch, South Africa Univ Cape Town Rondebosch South Africa ZA-7701 sch, South Africa
Citazione:
G. Gade et al., "Potencies of naturally-occurring AKH/RPCH peptides in Locusta migratoria in the acetate uptake assay in vitro and comparison with their potencies in the lipid mobilisation assay in vivo", ACT BIOL HU, 51(2-4), 2000, pp. 369-377

Abstract

The biological potencies of a number of naturally-occurring octa- and decapeptides of the large AKH/RPCH family of peptides were determined in Locusta migratoria using the lipid-mobilising assay in vivo and the acetate uptake assay in vitro. The most potent of the newly-tested peptides in the in vitro assay, Ph1-CC, differs from the endogenous major locust peptide, Lom-AKH-I, only by an exchange of serine versus threonine at position 10. However, the most active peptide in the ill vitro assay remains Lom-AKH-III. At the other extreme is the peptide Mem-CC which contains a tyrosine residue at position 4 rather than the more typical phenylalanine. This peptide is over20000 times less potent than LomAKH-III in the ill vitro assay, and also results in an unusual dose-response curve in the in vivo assay. Only a few peptides are approximately equipotent in both assays, but mostly the bioanalogues have a higher potency in vitro. The majority of them are 2- to 10-fold more potent in vitro, but Ani-AKH and LomAKH-III are 19- and 48-fold morepotent. The results are discussed in relation to either the actions of proteases or of possible preferential binding of different receptors involved in the different assays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 01:47:37