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Titolo:
Multi-epitope schistosome vaccine candidates tested for protective immunogenicity in mice
Autore:
Yang, W; Jackson, DC; Zeng, QR; McManus, DP;
Indirizzi:
Queensland Inst Med Res, Australian Ctr Int & Trop Hlth & Nutr, Mol Parasitol Unit, Brisbane, Qld 4029, Australia Queensland Inst Med Res Brisbane Qld Australia 4029 , Qld 4029, Australia Univ Queensland, Bancroft Ctr, Brisbane, Qld 4029, Australia Univ Queensland Brisbane Qld Australia 4029 Brisbane, Qld 4029, Australia Univ Melbourne, Dept Microbiol & Immunol, Cooperat Res Ctr Vaccine Technol, Parkville, Vic 3052, Australia Univ Melbourne Parkville Vic Australia 3052 arkville, Vic 3052, Australia
Titolo Testata:
VACCINE
fascicolo: 1, volume: 19, anno: 2000,
pagine: 103 - 113
SICI:
0264-410X(20000815)19:1<103:MSVCTF>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRIOSE-PHOSPHATE ISOMERASE; INTEGRAL MEMBRANE-PROTEIN; B-CELL EPITOPE; JAPONICUM PARAMYOSIN; TRIOSEPHOSPHATE ISOMERASE; NUCLEOTIDE-SEQUENCE; SYNTHETIC PEPTIDES; MOLECULAR-CLONING; MANSONI; ANTIGEN;
Keywords:
Schistosoma mansoni; synthetic peptide; polyepitope; polytope; polymer; DNA synthesis; expression; purification; immunisation; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: McManus, DP Queensland Inst Med Res, Australian Ctr Int & Trop Hlth & Nutr, Mol Parasitol Unit, 300 Herston Rd, Brisbane, Qld 4029, Australia Queensland Inst Med Res 300 Herston Rd Brisbane Qld Australia 4029
Citazione:
W. Yang et al., "Multi-epitope schistosome vaccine candidates tested for protective immunogenicity in mice", VACCINE, 19(1), 2000, pp. 103-113

Abstract

The major challenge in the development of anti-schistosome vaccines is to use defined antigens to stimulate an appropriate immune response that leadsto resistance. Several promising candidate vaccine antigens including the glycolytic enzyme triosephosphate isomerase (SmTPI), a 28 kDa glutathione-S-transferase (Sm28), the myofibrilar protein paramyosin (Sm97), an integralmembrane protein (Sm23) and calpain (Smcalpain) have been characterised and their primary sequences derived for Schistosoma mansoni. Furthermore, sequences are available For synthetic peptides mimicking epitopes on these molecules capable of inducing schistosmne-specific T- and B-cell responses. These schistosome vaccine candidates have generally been tested with varying degrees of success as single components, with only one report of the use ofa multivalent antigen or multi-epitope approach. We describe the assembly of multiple defined and different epitopes of S. mansoni into a variety of single covalent structures; these included a DNA vaccine encoding differentepitopes in tandem, the polyprotein itself that is encoded by this DNA acid branched synthetic peptide epitope-based polymers in which the individualepitopes are pendant from an inert backbone. Each of the vaccine constructs examined, with the exception of the DNA vaccine, generated antibodies that were capable of binding to a tandem sequence of the epitopes. Although these results were encouraging, none of the constructs protected animals fromsubsequent challenge infection, indicating that the immune responses elicited were inadequate or inappropriate for parasite killing in vivo. (C) 2000Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:30:48